Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4494237..4494855 Replicon chromosome
Accession NZ_CP031609
Organism Escherichia coli strain N3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AXA56_RS22265 Protein ID WP_001291435.1
Coordinates 4494637..4494855 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AXA56_RS22260 Protein ID WP_000344800.1
Coordinates 4494237..4494611 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXA56_RS22250 4489326..4490519 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AXA56_RS22255 4490542..4493691 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
AXA56_RS22260 4494237..4494611 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AXA56_RS22265 4494637..4494855 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AXA56_RS22270 4495027..4495578 + 552 WP_042003200.1 maltose O-acetyltransferase -
AXA56_RS22275 4495694..4496164 + 471 WP_000136192.1 YlaC family protein -
AXA56_RS22280 4496328..4497878 + 1551 WP_097178333.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AXA56_RS22285 4497920..4498273 - 354 WP_016246925.1 DUF1428 family protein -
AXA56_RS22295 4498652..4498963 + 312 WP_000409911.1 MGMT family protein -
AXA56_RS22300 4498994..4499566 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T109542 WP_001291435.1 NZ_CP031609:4494637-4494855 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T109542 NZ_CP031609:4494637-4494855 [Escherichia coli]
ATGTCCGAAAAACCTTTAACCAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT109542 WP_000344800.1 NZ_CP031609:4494237-4494611 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT109542 NZ_CP031609:4494237-4494611 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References