Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3425885..3426523 Replicon chromosome
Accession NZ_CP031609
Organism Escherichia coli strain N3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AXA56_RS16935 Protein ID WP_000813794.1
Coordinates 3426347..3426523 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AXA56_RS16930 Protein ID WP_001270286.1
Coordinates 3425885..3426301 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXA56_RS16910 3421037..3421978 - 942 WP_116836584.1 ABC transporter permease -
AXA56_RS16915 3421979..3422992 - 1014 WP_116836585.1 ABC transporter ATP-binding protein -
AXA56_RS16920 3423010..3424155 - 1146 WP_116836586.1 ABC transporter substrate-binding protein -
AXA56_RS16925 3424400..3425806 - 1407 WP_042002767.1 PLP-dependent aminotransferase family protein -
AXA56_RS16930 3425885..3426301 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AXA56_RS16935 3426347..3426523 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AXA56_RS16940 3426745..3426975 + 231 WP_000491567.1 DUF2554 family protein -
AXA56_RS16945 3427067..3429028 - 1962 WP_042002768.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AXA56_RS16950 3429101..3429637 - 537 WP_000429505.1 DNA-binding transcriptional regulator SutR -
AXA56_RS16955 3429690..3430898 + 1209 WP_116836587.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T109541 WP_000813794.1 NZ_CP031609:c3426523-3426347 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T109541 NZ_CP031609:c3426523-3426347 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT109541 WP_001270286.1 NZ_CP031609:c3426301-3425885 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT109541 NZ_CP031609:c3426301-3425885 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References