Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1897483..1898137 Replicon chromosome
Accession NZ_CP031609
Organism Escherichia coli strain N3

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AXA56_RS09420 Protein ID WP_000244781.1
Coordinates 1897730..1898137 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AXA56_RS09415 Protein ID WP_000354046.1
Coordinates 1897483..1897749 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXA56_RS09395 1893571..1895004 - 1434 WP_042004280.1 6-phospho-beta-glucosidase BglA -
AXA56_RS09400 1895049..1895360 + 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
AXA56_RS09405 1895524..1896183 + 660 WP_000250274.1 hemolysin III family protein -
AXA56_RS09410 1896260..1897240 - 981 WP_042004241.1 tRNA-modifying protein YgfZ -
AXA56_RS09415 1897483..1897749 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AXA56_RS09420 1897730..1898137 + 408 WP_000244781.1 protein YgfX Toxin
AXA56_RS09425 1898177..1898698 - 522 WP_001055874.1 flavodoxin FldB -
AXA56_RS09430 1898810..1899706 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
AXA56_RS09435 1899731..1900441 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AXA56_RS09440 1900447..1902180 + 1734 WP_042004240.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T109531 WP_000244781.1 NZ_CP031609:1897730-1898137 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T109531 NZ_CP031609:1897730-1898137 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGTGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT109531 WP_000354046.1 NZ_CP031609:1897483-1897749 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT109531 NZ_CP031609:1897483-1897749 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References