Detailed information of TA system TA109528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1314859..1315454
• Replicon: chromosome
• Accession: NZ_CP031609
• Organism: Escherichia coli strain N3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: AXA56_RS06305
• Protein ID: WP_000155159.1
• Coordinates: 1315077..1315454 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: AXA56_RS06300
• Protein ID: WP_000557315.1
• Coordinates: 1314859..1315080 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA56_RS06275	1309935..1311044	+	1110	WP_116836237.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AXA56_RS06280	1311092..1312018	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AXA56_RS06285	1312015..1313292	+	1278	WP_116836238.1	branched chain amino acid ABC transporter permease LivM	-
AXA56_RS06290	1313289..1314056	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AXA56_RS06295	1314058..1314771	+	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
AXA56_RS06300	1314859..1315080	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AXA56_RS06305	1315077..1315454	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AXA56_RS06310	1315663..1316979	+	1317	WP_000803190.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
AXA56_RS06315	1317152..1318039	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
AXA56_RS06320	1318036..1318881	+	846	WP_032352414.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
AXA56_RS06325	1318883..1319953	+	1071	WP_042002817.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1304176..1320693	16517

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T109528 WP_000155159.1 NZ_CP031609:1315077-1315454 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T109528 NZ_CP031609:1315077-1315454 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCAGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT109528 WP_000557315.1 NZ_CP031609:1314859-1315080 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT109528 NZ_CP031609:1314859-1315080 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGATG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32