Detailed information of TA system TA109527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1136877..1137489
• Replicon: chromosome
• Accession: NZ_CP031609
• Organism: Escherichia coli strain N3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: AXA56_RS05415
• Protein ID: WP_000833473.1
• Coordinates: 1136877..1137062 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: AXA56_RS05420
• Protein ID: WP_000499744.1
• Coordinates: 1137079..1137489 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA56_RS05395	1132392..1133543	+	1152	WP_116836206.1	L-threonine dehydrogenase	-
AXA56_RS05400	1133744..1135282	+	1539	WP_000183978.1	aldehyde dehydrogenase AldB	-
AXA56_RS05405	1135323..1136402	-	1080	WP_000061477.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
AXA56_RS05415	1136877..1137062	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
AXA56_RS05420	1137079..1137489	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AXA56_RS05425	1137561..1139525	-	1965	WP_116836207.1	glycoside hydrolase family 127 protein	-
AXA56_RS05430	1139536..1140936	-	1401	WP_116836208.1	MFS transporter	-
AXA56_RS05435	1141162..1141977	+	816	WP_042002931.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T109527 WP_000833473.1 NZ_CP031609:1136877-1137062 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T109527 NZ_CP031609:1136877-1137062 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT109527 WP_000499744.1 NZ_CP031609:1137079-1137489 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT109527 NZ_CP031609:1137079-1137489 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCTGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAATAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9