Detailed information of TA system TA109525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 374744..375339
• Replicon: chromosome
• Accession: NZ_CP031609
• Organism: Escherichia coli strain N3

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: AXA56_RS01710
• Protein ID: WP_000239581.1
• Coordinates: 374744..375094 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: V0YZQ7
• Locus tag: AXA56_RS01715
• Protein ID: WP_001223206.1
• Coordinates: 375088..375339 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA56_RS01690	370198..371220	-	1023	WP_160117233.1	ABC transporter permease	-
AXA56_RS01695	371234..372736	-	1503	WP_116836116.1	sugar ABC transporter ATP-binding protein	-
AXA56_RS01700	372869..373825	-	957	WP_000265932.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
AXA56_RS01705	374135..374665	+	531	WP_000055075.1	inorganic diphosphatase	-
AXA56_RS01710	374744..375094	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
AXA56_RS01715	375088..375339	-	252	WP_001223206.1	type II toxin-antitoxin system ChpS family antitoxin	Antitoxin
AXA56_RS01720	375551..375892	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
AXA56_RS01725	375895..379674	-	3780	WP_001577602.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T109525 WP_000239581.1 NZ_CP031609:c375094-374744 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T109525 NZ_CP031609:c375094-374744 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCGGGATTCAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9303.62 Da
• Isoelectric Point: 4.6509

>AT109525 WP_001223206.1 NZ_CP031609:c375339-375088 [Escherichia coli]
MRITIKKWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT109525 NZ_CP031609:c375339-375088 [Escherichia coli]
ATGCGTATTACCATAAAAAAATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: V0YZQ7