Detailed information of TA system TA109517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3626409..3628000
• Replicon: chromosome
• Accession: NZ_CP031606
• Organism: Pseudomonas sp. phDV1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W6QYA1
• Locus tag: DZC76_RS17555
• Protein ID: WP_003459717.1
• Coordinates: 3626675..3628000 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: W6RJ44
• Locus tag: DZC76_RS17550
• Protein ID: WP_003459718.1
• Coordinates: 3626409..3626675 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZC76_RS17525	3622584..3623387	-	804	WP_003459721.1	IS21-like element ISPst3 family helper ATPase IstB	-
DZC76_RS17530	3623380..3624879	-	1500	WP_116620715.1	IS21 family transposase	-
DZC76_RS17540	3624997..3625320	+	324	Protein_3353	integrase	-
DZC76_RS17550	3626409..3626675	-	267	WP_003459718.1	helix-turn-helix domain-containing protein	Antitoxin
DZC76_RS17555	3626675..3628000	-	1326	WP_003459717.1	HipA domain-containing protein	Toxin
DZC76_RS17560	3628273..3628653	-	381	Protein_3356	IS66 family transposase	-
DZC76_RS17565	3628673..3629029	-	357	WP_116620717.1	IS66 family insertion sequence element accessory protein TnpB	-
DZC76_RS17570	3629321..3629776	-	456	WP_003459711.1	hypothetical protein	-
DZC76_RS17575	3629792..3630574	-	783	WP_003459710.1	amidohydrolase	-
DZC76_RS17580	3630648..3631796	-	1149	WP_003459709.1	pyridoxal phosphate-dependent aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3616013..3628000	11987
	-	inside	IScluster/Tn	-	-	3622584..3629032	6448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-385)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 49143.75 Da
• Isoelectric Point: 4.8860

>T109517 WP_003459717.1 NZ_CP031606:c3628000-3626675 [Pseudomonas sp. phDV1]
MHNLTLQVYRDGQWRNAVELRFEQPEEGLEGACAYGYQHGYLVDSLESVGTRLNVAVSASYPLGWDSWRDKKAPAFLFDI
LPSGAARRFLLKRLGGERPEALNLDLYLLGRCTPAPIGNLRIKEAVEAIAGSPMLGFTRDEVISRDSRFLEYAYEQGAAI
GGATGAGGEAPKLLLTEDRQGALHPDAVLPDADAAQHWFVKFARNKASQTDQDILRSEYCFYRAVQQLGMDTVAAEGLAL
EEGNKPSLWMHRFDREISPAGVCRTAVESMYSLCGVTEAGSYMLHTQVLGKLVELWRAVGQEQQVGDLVFEYMRRDLLNQ
ILGNSDNHGRNTAILRSEQGVRLAPIYDLAPMVMDEEGVTRTTKWPKHIELAGEVNWRAACDEVAEWIDADELFQRLQLA
AQDLLALPDLLSAQGLPDSTMSHPRIALRNLQQRMLQWGLV

Nucleotide

• Length: 1326 bp

>T109517 NZ_CP031606:c3628000-3626675 [Pseudomonas sp. phDV1]
ATGCACAATCTTACTCTGCAGGTTTATAGGGATGGCCAGTGGCGCAATGCCGTGGAACTCCGTTTCGAGCAGCCCGAGGA
AGGGCTGGAGGGTGCTTGTGCTTATGGCTACCAGCATGGCTATCTGGTTGACTCATTGGAGTCTGTAGGCACTCGGCTGA
ATGTGGCTGTCAGTGCATCTTACCCTCTCGGATGGGATTCTTGGCGAGATAAGAAGGCGCCTGCGTTTCTCTTCGACATC
CTGCCATCAGGTGCAGCAAGACGCTTTCTACTGAAGCGTTTGGGCGGCGAGCGGCCCGAGGCTCTCAATCTTGATCTCTA
CCTTCTTGGGCGATGCACACCTGCGCCAATTGGCAATCTGCGTATCAAGGAGGCTGTCGAGGCGATTGCGGGCAGTCCGA
TGTTGGGTTTCACACGCGATGAGGTTATTAGCCGGGACTCGCGATTCCTTGAGTACGCGTATGAGCAGGGCGCAGCCATT
GGCGGGGCGACAGGTGCTGGCGGTGAGGCCCCCAAACTGTTGCTGACTGAAGATCGGCAGGGCGCCTTACATCCAGATGC
CGTGCTGCCAGATGCAGACGCCGCCCAGCACTGGTTTGTGAAGTTCGCGCGTAACAAAGCCAGTCAGACCGATCAGGACA
TCCTGCGCAGCGAGTATTGCTTCTATCGTGCCGTCCAGCAGCTCGGAATGGACACGGTTGCAGCTGAAGGGCTGGCATTG
GAGGAGGGCAATAAGCCCAGTCTCTGGATGCATCGCTTTGATCGGGAGATTAGCCCCGCTGGGGTTTGCCGAACTGCCGT
TGAGTCAATGTACTCGTTATGTGGCGTAACCGAGGCGGGTAGTTACATGCTGCACACGCAAGTACTGGGGAAGCTGGTCG
AGCTATGGCGAGCTGTTGGGCAGGAACAGCAGGTCGGTGATCTGGTGTTTGAATACATGCGGCGTGATCTGCTGAACCAG
ATCCTCGGAAATTCAGACAACCACGGACGTAACACCGCGATTCTCCGCTCCGAGCAGGGGGTTCGGTTGGCGCCAATCTA
CGACCTGGCTCCTATGGTCATGGACGAGGAGGGTGTTACGCGTACGACGAAGTGGCCAAAGCACATCGAGCTTGCAGGAG
AGGTCAACTGGAGAGCGGCCTGTGATGAGGTCGCAGAGTGGATTGATGCCGATGAACTGTTTCAACGATTGCAGTTGGCA
GCTCAGGACTTGCTCGCGCTACCTGACTTGCTGAGTGCACAAGGCCTTCCTGATTCAACCATGAGTCACCCGCGTATAGC
CCTGAGAAATCTTCAGCAGCGGATGCTTCAGTGGGGGTTGGTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9933.55 Da
• Isoelectric Point: 10.9502

>AT109517 WP_003459718.1 NZ_CP031606:c3626675-3626409 [Pseudomonas sp. phDV1]
MDILERNRILNELQQQLASGEITIGGAVRRLRKEVTGLQQARFAQMCKLSLRALRQLELDESNPTVQTLNSVFNPFGMQV
GIVPKTPR

Nucleotide

• Length: 267 bp

>AT109517 NZ_CP031606:c3626675-3626409 [Pseudomonas sp. phDV1]
ATGGACATTCTTGAGCGCAATCGCATTTTGAATGAGCTGCAGCAGCAGTTGGCTAGCGGCGAGATCACGATTGGAGGAGC
AGTGCGACGCCTGCGTAAGGAAGTGACTGGCTTGCAGCAGGCCAGATTTGCACAGATGTGCAAACTGTCATTGCGGGCCT
TGCGACAGCTTGAGTTGGATGAGAGCAATCCGACGGTTCAGACATTGAACAGTGTGTTTAATCCCTTTGGCATGCAGGTC
GGCATAGTGCCGAAGACTCCGCGTTGA

Structures

Toxin

• AlphaFold DB: W6QYA1

Antitoxin

• AlphaFold DB: W6RJ44