Detailed information of TA system TA109512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 885666..886234
• Replicon: chromosome
• Accession: NZ_CP031606
• Organism: Pseudomonas sp. phDV1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: W6QTT6
• Locus tag: DZC76_RS04035
• Protein ID: WP_004424733.1
• Coordinates: 885666..885947 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: W6RBZ2
• Locus tag: DZC76_RS04040
• Protein ID: WP_004424730.1
• Coordinates: 885944..886234 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZC76_RS04030	883006..885663	+	2658	WP_116617931.1	CRISPR-associated helicase Cas3'	-
DZC76_RS04035	885666..885947	-	282	WP_004424733.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
DZC76_RS04040	885944..886234	-	291	WP_004424730.1	hypothetical protein	Antitoxin
DZC76_RS04045	886500..886751	+	252	WP_004424728.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DZC76_RS04050	886751..887176	+	426	WP_084340296.1	type II toxin-antitoxin system VapC family toxin	-
DZC76_RS04055	887286..888821	+	1536	WP_004424723.1	type I-E CRISPR-associated protein Cse1/CasA	-
DZC76_RS04060	888818..889360	+	543	WP_004424722.1	type I-E CRISPR-associated protein Cse2/CasB	-
DZC76_RS04065	889371..890513	+	1143	WP_116617932.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
DZC76_RS04070	890516..891178	+	663	WP_116617933.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10565.07 Da
• Isoelectric Point: 8.6765

>T109512 WP_004424733.1 NZ_CP031606:c885947-885666 [Pseudomonas sp. phDV1]
VKLAWTRLALNDRQAIRSYIAQDNPIATLALDELFTEKASRLADHPGLGRPGRVSGTRELVVHQHDLMIYDLVNDQVRIL
RVLHTARQWPSAD

Nucleotide

• Length: 282 bp

>T109512 NZ_CP031606:c885947-885666 [Pseudomonas sp. phDV1]
GTGAAACTGGCCTGGACGCGTCTTGCGCTGAACGACCGCCAAGCCATCCGCAGTTATATCGCCCAGGACAATCCCATTGC
CACCCTGGCGCTGGATGAATTGTTCACGGAGAAGGCCAGCCGCCTGGCCGATCATCCCGGCTTGGGGCGACCAGGCCGGG
TAAGCGGCACTCGCGAACTGGTTGTGCATCAGCACGATCTGATGATCTACGACCTGGTCAATGACCAAGTACGGATACTC
CGCGTGTTGCATACGGCCCGCCAGTGGCCAAGTGCAGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10878.08 Da
• Isoelectric Point: 4.7266

>AT109512 WP_004424730.1 NZ_CP031606:c886234-885944 [Pseudomonas sp. phDV1]
MSEATFTFRVDDALKSEFAQAAKARDRTGAQLLRDFMRDFVRQQQEAAEHDVWLRRQVQMGLDSANAGELLSSEEVEAEA
AAWRAEMRRKLDSAAS

Nucleotide

• Length: 291 bp

>AT109512 NZ_CP031606:c886234-885944 [Pseudomonas sp. phDV1]
ATGAGTGAAGCCACCTTTACCTTTCGCGTAGACGACGCCCTGAAAAGCGAATTTGCCCAGGCAGCCAAGGCCCGCGACCG
TACCGGCGCGCAGTTGCTGCGCGACTTCATGCGCGACTTCGTCCGGCAGCAGCAAGAGGCCGCCGAGCACGATGTCTGGC
TGCGCCGCCAGGTACAGATGGGGCTGGATTCGGCCAATGCCGGTGAGTTGCTCAGCAGCGAAGAGGTCGAAGCCGAAGCC
GCCGCATGGCGCGCTGAAATGCGCCGCAAGCTGGACAGCGCCGCGTCGTGA

Structures

Toxin

• AlphaFold DB: W6QTT6

Antitoxin

• AlphaFold DB: W6RBZ2