Detailed information of TA system TA109510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2645831..2646366
• Replicon: chromosome
• Accession: NZ_CP031605
• Organism: Staphylococcus pseudintermedius strain VB16

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DRC71_RS12775
• Protein ID: WP_096533788.1
• Coordinates: 2646028..2646366 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A2A4GVU0
• Locus tag: DRC71_RS12770
• Protein ID: WP_051006000.1
• Coordinates: 2645831..2646031 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRC71_RS12750 (DRC71_12850)	2641666..2643051	+	1386	WP_189695512.1	recombinase family protein	-
DRC71_RS12755 (DRC71_12855)	2643070..2643711	-	642	Protein_2523	SLC13 family permease	-
DRC71_RS12760 (DRC71_12860)	2644291..2644752	-	462	WP_014614683.1	threonine/serine exporter family protein	-
DRC71_RS12765 (DRC71_12865)	2644765..2645520	-	756	WP_014614684.1	threonine/serine exporter family protein	-
DRC71_RS12770 (DRC71_12870)	2645831..2646031	+	201	WP_051006000.1	AbrB family transcriptional regulator	Antitoxin
DRC71_RS12775 (DRC71_12875)	2646028..2646366	+	339	WP_096533788.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DRC71_RS12780 (DRC71_12880)	2646578..2647333	-	756	WP_096533787.1	heme-dependent peroxidase	-
DRC71_RS12785 (DRC71_12885)	2647501..2648490	+	990	WP_014614688.1	phosphate acetyltransferase	-
DRC71_RS12790 (DRC71_12890)	2648490..2649326	+	837	WP_014614689.1	lipoate--protein ligase family protein	-
DRC71_RS12795 (DRC71_12895)	2649442..2649846	-	405	WP_096533786.1	arsenate reductase (thioredoxin)	-
DRC71_RS12800 (DRC71_12900)	2649862..2651151	-	1290	WP_015728657.1	arsenite efflux transporter membrane subunit ArsB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13027.15 Da
• Isoelectric Point: 9.6920

>T109510 WP_096533788.1 NZ_CP031605:2646028-2646366 [Staphylococcus pseudintermedius]
MSVKQFDIIYIDLNPTRGREKQNIRPCLVINNQIMIDGTEFVWVVPIINRELRYPTDIELKTKKGLVSGVIDTIQIRALD
LNARQHNYKDALQDNLKSKVLKAIQTYLNPNY

Nucleotide

• Length: 339 bp

>T109510 NZ_CP031605:2646028-2646366 [Staphylococcus pseudintermedius]
ATGAGTGTTAAACAATTTGACATCATTTACATTGATTTAAATCCAACTCGCGGTCGTGAAAAACAAAACATAAGACCTTG
TTTAGTGATCAATAACCAAATAATGATTGACGGCACTGAATTTGTATGGGTTGTACCTATCATAAATCGTGAGTTGCGTT
ATCCAACCGATATAGAACTTAAAACCAAAAAAGGACTGGTATCTGGCGTGATAGATACGATTCAAATACGTGCTTTAGAT
TTAAACGCTCGCCAACATAACTATAAAGATGCATTACAAGACAATCTAAAAAGTAAAGTTTTAAAAGCAATACAAACATA
TTTAAATCCAAACTACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7375.24 Da
• Isoelectric Point: 4.3623

>AT109510 WP_051006000.1 NZ_CP031605:2645831-2646031 [Staphylococcus pseudintermedius]
MSLSKKTIQSVDFEIGDTVEVQKINGGLFITKKEVSIEDSIKNFFQNGGKYQESEVDFGESVGREI

Nucleotide

• Length: 201 bp

>AT109510 NZ_CP031605:2645831-2646031 [Staphylococcus pseudintermedius]
ATGAGTTTAAGTAAAAAAACGATTCAATCCGTTGATTTTGAGATTGGCGATACAGTTGAAGTACAAAAAATAAATGGCGG
GCTGTTTATTACTAAAAAAGAAGTATCTATTGAAGATAGTATCAAAAACTTCTTCCAAAATGGTGGTAAGTATCAAGAAT
CAGAAGTAGACTTCGGCGAAAGTGTGGGTCGTGAAATATGA

Structures

Antitoxin

• AlphaFold DB: A0A2A4GVU0