Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1947187..1947711 Replicon chromosome
Accession NZ_CP031603
Organism Staphylococcus pseudintermedius strain AK9

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag DNI27_RS08970 Protein ID WP_014613379.1
Coordinates 1947358..1947711 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag DNI27_RS08965 Protein ID WP_014613378.1
Coordinates 1947187..1947357 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNI27_RS08940 (DNI27_09105) 1943076..1943549 + 474 WP_014613373.1 PH domain-containing protein -
DNI27_RS08945 (DNI27_09110) 1943542..1945071 + 1530 WP_096533708.1 PH domain-containing protein -
DNI27_RS08950 (DNI27_09115) 1945055..1945573 + 519 WP_065354119.1 PH domain-containing protein -
DNI27_RS08955 (DNI27_09120) 1945570..1945920 + 351 WP_014613376.1 holo-ACP synthase -
DNI27_RS08960 (DNI27_09125) 1945954..1947102 + 1149 WP_014613377.1 alanine racemase -
DNI27_RS08965 (DNI27_09130) 1947187..1947357 + 171 WP_014613378.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
DNI27_RS08970 (DNI27_09135) 1947358..1947711 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DNI27_RS08975 (DNI27_09140) 1947769..1948773 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
DNI27_RS08980 (DNI27_09145) 1948852..1949178 + 327 WP_014613381.1 anti-sigma factor antagonist -
DNI27_RS08985 (DNI27_09150) 1949178..1949657 + 480 WP_031253870.1 anti-sigma B factor RsbW -
DNI27_RS08990 (DNI27_09155) 1949632..1950402 + 771 WP_015729428.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T109503 WP_014613379.1 NZ_CP031603:1947358-1947711 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T109503 NZ_CP031603:1947358-1947711 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT109503 WP_014613378.1 NZ_CP031603:1947187-1947357 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT109503 NZ_CP031603:1947187-1947357 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References