Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1930938..1931459 Replicon chromosome
Accession NZ_CP031603
Organism Staphylococcus pseudintermedius strain AK9

Toxin (Protein)


Gene name TsdT Uniprot ID A0A3D8YLE8
Locus tag DNI27_RS08890 Protein ID WP_014613364.1
Coordinates 1931238..1931459 (+) Length 74 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A166R1D0
Locus tag DNI27_RS08885 Protein ID WP_014613363.1
Coordinates 1930938..1931225 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNI27_RS08860 (DNI27_09025) 1926199..1927002 + 804 WP_014613358.1 hydroxyethylthiazole kinase -
DNI27_RS08865 (DNI27_09030) 1926992..1927630 + 639 WP_014613359.1 thiamine phosphate synthase -
DNI27_RS08870 (DNI27_09035) 1927719..1928585 + 867 WP_014613360.1 membrane protein insertase YidC -
DNI27_RS08875 (DNI27_09040) 1928639..1929295 - 657 WP_014613361.1 HD domain-containing protein -
DNI27_RS08880 (DNI27_09045) 1929316..1930806 - 1491 WP_014613362.1 cardiolipin synthase -
DNI27_RS08885 (DNI27_09050) 1930938..1931225 + 288 WP_014613363.1 copper-sensing transcriptional repressor CsoR Antitoxin
DNI27_RS08890 (DNI27_09055) 1931238..1931459 + 222 WP_014613364.1 heavy metal-associated domain-containing protein Toxin
DNI27_RS08895 (DNI27_09060) 1931463..1931669 + 207 WP_014613365.1 cation transporter -
DNI27_RS08900 (DNI27_09065) 1931770..1933950 + 2181 WP_037542539.1 heavy metal translocating P-type ATPase -
DNI27_RS08905 1934036..1934182 + 147 WP_014613367.1 Lmo0850 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(21-64)

Antitoxin

Metal-sensitive transcriptional repressor

(3-93)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8065.10 Da        Isoelectric Point: 4.3480

>T109502 WP_014613364.1 NZ_CP031603:1931238-1931459 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGFPVIQSHKGV

Download         Length: 222 bp

>T109502 NZ_CP031603:1931238-1931459 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10997.59 Da        Isoelectric Point: 7.0504

>AT109502 WP_014613363.1 NZ_CP031603:1930938-1931225 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Download         Length: 288 bp

>AT109502 NZ_CP031603:1930938-1931225 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3D8YLE8

Antitoxin

Source ID Structure
AlphaFold DB A0A166R1D0

References