Detailed information of TA system TA109500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 712029..712564
• Replicon: chromosome
• Accession: NZ_CP031603
• Organism: Staphylococcus pseudintermedius strain AK9

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DNI27_RS03350
• Protein ID: WP_096533788.1
• Coordinates: 712226..712564 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A2A4GVU0
• Locus tag: DNI27_RS03345
• Protein ID: WP_051006000.1
• Coordinates: 712029..712229 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNI27_RS03320 (DNI27_03405)	707294..707899	-	606	WP_110161166.1	DUF2812 domain-containing protein	-
DNI27_RS03325 (DNI27_03410)	707883..708215	-	333	WP_110161165.1	PadR family transcriptional regulator	-
DNI27_RS03330 (DNI27_03415)	708368..709909	-	1542	WP_096533789.1	SLC13 family permease	-
DNI27_RS03335 (DNI27_03420)	710489..710950	-	462	WP_014614683.1	threonine/serine exporter family protein	-
DNI27_RS03340 (DNI27_03425)	710963..711718	-	756	WP_014614684.1	threonine/serine exporter family protein	-
DNI27_RS03345 (DNI27_03430)	712029..712229	+	201	WP_051006000.1	AbrB family transcriptional regulator	Antitoxin
DNI27_RS03350 (DNI27_03435)	712226..712564	+	339	WP_096533788.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DNI27_RS03355 (DNI27_03440)	712776..713531	-	756	WP_096533787.1	heme-dependent peroxidase	-
DNI27_RS03360 (DNI27_03445)	713699..714688	+	990	WP_014614688.1	phosphate acetyltransferase	-
DNI27_RS03365 (DNI27_03450)	714688..715524	+	837	WP_014614689.1	lipoate--protein ligase family protein	-
DNI27_RS03370 (DNI27_03455)	715640..716044	-	405	WP_096533786.1	arsenate reductase (thioredoxin)	-
DNI27_RS03375 (DNI27_03460)	716060..717349	-	1290	WP_015728657.1	arsenite efflux transporter membrane subunit ArsB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13027.15 Da
• Isoelectric Point: 9.6920

>T109500 WP_096533788.1 NZ_CP031603:712226-712564 [Staphylococcus pseudintermedius]
MSVKQFDIIYIDLNPTRGREKQNIRPCLVINNQIMIDGTEFVWVVPIINRELRYPTDIELKTKKGLVSGVIDTIQIRALD
LNARQHNYKDALQDNLKSKVLKAIQTYLNPNY

Nucleotide

• Length: 339 bp

>T109500 NZ_CP031603:712226-712564 [Staphylococcus pseudintermedius]
ATGAGTGTTAAACAATTTGACATCATTTACATTGATTTAAATCCAACTCGCGGTCGTGAAAAACAAAACATAAGACCTTG
TTTAGTGATCAATAACCAAATAATGATTGACGGCACTGAATTTGTATGGGTTGTACCTATCATAAATCGTGAGTTGCGTT
ATCCAACCGATATAGAACTTAAAACCAAAAAAGGACTGGTATCTGGCGTGATAGATACGATTCAAATACGTGCTTTAGAT
TTAAACGCTCGCCAACATAACTATAAAGATGCATTACAAGACAATCTAAAAAGTAAAGTTTTAAAAGCAATACAAACATA
TTTAAATCCAAACTACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7375.24 Da
• Isoelectric Point: 4.3623

>AT109500 WP_051006000.1 NZ_CP031603:712029-712229 [Staphylococcus pseudintermedius]
MSLSKKTIQSVDFEIGDTVEVQKINGGLFITKKEVSIEDSIKNFFQNGGKYQESEVDFGESVGREI

Nucleotide

• Length: 201 bp

>AT109500 NZ_CP031603:712029-712229 [Staphylococcus pseudintermedius]
ATGAGTTTAAGTAAAAAAACGATTCAATCCGTTGATTTTGAGATTGGCGATACAGTTGAAGTACAAAAAATAAATGGCGG
GCTGTTTATTACTAAAAAAGAAGTATCTATTGAAGATAGTATCAAAAACTTCTTCCAAAATGGTGGTAAGTATCAAGAAT
CAGAAGTAGACTTCGGCGAAAGTGTGGGTCGTGAAATATGA

Structures

Antitoxin

• AlphaFold DB: A0A2A4GVU0