Detailed information of TA system TA109496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 136983..137719
• Replicon: plasmid pIncFII-1502320
• Accession: NZ_CP031583
• Organism: Klebsiella pneumoniae strain N4b

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AXA58_RS29670
• Protein ID: WP_003026803.1
• Coordinates: 137237..137719 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AXA58_RS29665
• Protein ID: WP_003026799.1
• Coordinates: 136983..137249 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA58_RS29620	133045..133407	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
AXA58_RS29625	133457..133807	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
AXA58_RS29630	134165..134434	+	270	WP_004152102.1	hypothetical protein	-
AXA58_RS29635	134422..134997	+	576	WP_004152103.1	hypothetical protein	-
AXA58_RS29640	135028..135522	+	495	WP_004152104.1	DNA-binding protein	-
AXA58_RS29645	135566..135934	+	369	WP_004152105.1	hypothetical protein	-
AXA58_RS29650	135968..136171	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
AXA58_RS29655	136220..136477	+	258	WP_004152107.1	hypothetical protein	-
AXA58_RS29660	136553..136807	+	255	WP_004152108.1	hypothetical protein	-
AXA58_RS29665	136983..137249	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AXA58_RS29670	137237..137719	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AXA58_RS29675	137878..139410	-	1533	WP_004118819.1	IS3 family transposase	-
AXA58_RS29680	139522..140925	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
AXA58_RS29685	140954..141586	-	633	WP_001567369.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(D)	-	1..198719	198719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T109496 WP_003026803.1 NZ_CP031583:137237-137719 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T109496 NZ_CP031583:137237-137719 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT109496 WP_003026799.1 NZ_CP031583:136983-137249 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT109496 NZ_CP031583:136983-137249 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66