Detailed information of TA system TA109493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 16075..16718
• Replicon: plasmid pIncFIA-1502320
• Accession: NZ_CP031581
• Organism: Klebsiella pneumoniae strain N4b

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: AXA58_RS27430
• Protein ID: WP_001044770.1
• Coordinates: 16075..16491 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AXA58_RS27435
• Protein ID: WP_001261282.1
• Coordinates: 16488..16718 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA58_RS27390	11446..11781	-	336	WP_000046891.1	thermonuclease family protein	-
AXA58_RS27400	12023..12586	+	564	WP_001515734.1	transposase	-
AXA58_RS27405	12651..13025	+	375	WP_001348195.1	cupin domain-containing protein	-
AXA58_RS27410	13049..13612	+	564	WP_001097412.1	chlorite dismutase family protein	-
AXA58_RS27415	13863..14960	-	1098	Protein_17	IS66 family transposase	-
AXA58_RS27420	15014..15718	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
AXA58_RS27425	15765..16001	-	237	WP_001348075.1	AAA family ATPase	-
AXA58_RS27430	16075..16491	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
AXA58_RS27435	16488..16718	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AXA58_RS31800	16675..17136	+	462	WP_185940046.1	hypothetical protein	-
AXA58_RS27445	17280..17630	+	351	WP_000493378.1	hypothetical protein	-
AXA58_RS27450	17681..18424	+	744	WP_004098973.1	hypothetical protein	-
AXA58_RS27455	18421..19197	+	777	WP_023302478.1	site-specific integrase	-
AXA58_RS27460	19255..19512	-	258	WP_000764642.1	hypothetical protein	-
AXA58_RS31805	19641..19744	-	104	Protein_27	hypothetical protein	-
AXA58_RS27465	20277..21143	+	867	WP_023302476.1	replication initiation protein	-
AXA58_RS27470	21250..21489	-	240	Protein_29	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..71335	71335

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T109493 WP_001044770.1 NZ_CP031581:c16491-16075 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T109493 NZ_CP031581:c16491-16075 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT109493 WP_001261282.1 NZ_CP031581:c16718-16488 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT109493 NZ_CP031581:c16718-16488 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3