Detailed information of TA system TA109492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 13869..14779
• Replicon: plasmid p1502320-3
• Accession: NZ_CP031580
• Organism: Klebsiella pneumoniae strain N4b

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2N4Z2R0
• Locus tag: AXA58_RS26930
• Protein ID: WP_004197882.1
• Coordinates: 14309..14779 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: AXA58_RS26925
• Protein ID: WP_004181895.1
• Coordinates: 13869..14312 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA58_RS26895	9024..9239	-	216	Protein_10	IS200/IS605 family transposase	-
AXA58_RS26900	9241..9810	+	570	Protein_11	transposase	-
AXA58_RS26910	10502..11989	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
AXA58_RS26915	12075..12497	+	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
AXA58_RS26920	12497..13768	+	1272	WP_004197886.1	translesion error-prone DNA polymerase V subunit UmuC	-
AXA58_RS26925	13869..14312	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
AXA58_RS26930	14309..14779	+	471	WP_004197882.1	RES family NAD+ phosphorylase	Toxin
AXA58_RS26935	14889..15149	-	261	WP_004026352.1	hypothetical protein	-
AXA58_RS26945	15839..17207	+	1369	Protein_18	IS3-like element ISKpn8 family transposase	-
AXA58_RS26950	17226..18338	-	1113	WP_014386573.1	IS481-like element ISKpn28 family transposase	-
AXA58_RS26955	18410..18568	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86800	86800
	-	flank	IS/Tn	-	-	9457..9810	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17633.92 Da
• Isoelectric Point: 4.5152

>T109492 WP_004197882.1 NZ_CP031580:14309-14779 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T109492 NZ_CP031580:14309-14779 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTTTTTTACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT109492 WP_004181895.1 NZ_CP031580:13869-14312 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT109492 NZ_CP031580:13869-14312 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2N4Z2R0

Antitoxin

• AlphaFold DB: A0A2J4RLY9