Detailed information of TA system TA109476

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1291934..1292640
• Replicon: chromosome
• Accession: NZ_CP031577
• Organism: Klebsiella pneumoniae strain N4b

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: AXA58_RS06595
• Protein ID: WP_024622903.1
• Coordinates: 1292272..1292640 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A485WAM6
• Locus tag: AXA58_RS06590
• Protein ID: WP_023302278.1
• Coordinates: 1291934..1292251 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA58_RS06545	1287075..1287899	+	825	WP_023302271.1	DUF945 domain-containing protein	-
AXA58_RS06550	1288108..1288818	+	711	WP_023302272.1	DeoR family transcriptional regulator	-
AXA58_RS06555	1288844..1289380	+	537	WP_023302273.1	DUF4339 domain-containing protein	-
AXA58_RS06560	1289422..1289859	+	438	WP_023301392.1	hypothetical protein	-
AXA58_RS06565	1289926..1290336	+	411	WP_023302274.1	hypothetical protein	-
AXA58_RS06570	1290414..1290650	+	237	WP_032410024.1	DUF905 domain-containing protein	-
AXA58_RS06575	1290737..1291195	+	459	WP_023302275.1	antirestriction protein	-
AXA58_RS06580	1291204..1291686	+	483	WP_023302276.1	RadC family protein	-
AXA58_RS06585	1291695..1291916	+	222	WP_023302277.1	DUF987 domain-containing protein	-
AXA58_RS06590	1291934..1292251	+	318	WP_023302278.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AXA58_RS06595	1292272..1292640	+	369	WP_024622903.1	TA system toxin CbtA family protein	Toxin
AXA58_RS06600	1292637..1292978	+	342	WP_024622904.1	hypothetical protein	-
AXA58_RS06605	1293101..1295746	-	2646	WP_024622905.1	LuxR family transcriptional regulator	-
AXA58_RS06610	1296119..1297078	+	960	WP_023302280.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.82 Da
• Isoelectric Point: 7.2897

>T109476 WP_024622903.1 NZ_CP031577:1292272-1292640 [Klebsiella pneumoniae]
MKNLPATISQAAKPCLSPVAVWQMLLTHLLEQHYGLMLSDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRYSYREVSNIVLSRSRI

Nucleotide

• Length: 369 bp

>T109476 NZ_CP031577:1292272-1292640 [Klebsiella pneumoniae]
ATGAAAAATCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCATCTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGTGATGAAGCCGTTATACAGGAACATATTG
ATGCCGGTATCACTCTGGCTAATGCTGTTAACTTTCTGGTGGAGAAGTATGAGTTAGTCCGTATCGATCGCTGTGGATTT
TCCTCACAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCATGCCAGGAAAGCGTGCGGGCTGATGAGCCGGTA
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGATATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11860.67 Da
• Isoelectric Point: 6.4629

>AT109476 WP_023302278.1 NZ_CP031577:1291934-1292251 [Klebsiella pneumoniae]
MSNPTWGLLRDITPCLGARLVQEGNRLYYLVDRASITGKFSDAEILKLDVVFPHFISQMESMLTIGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT109476 NZ_CP031577:1291934-1292251 [Klebsiella pneumoniae]
ATGAGCAACCCCACATGGGGCCTGCTGCGGGATATCACGCCGTGCCTAGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GTACTATCTGGTTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAATCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGCTGACCATTGGTGAAATGAATCCCCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCTCACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A485WAM6