Detailed information of TA system TA109454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4616995..4617611
• Replicon: chromosome
• Accession: NZ_CP031571
• Organism: Enterobacter hormaechei strain N1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AXA51_RS22650
• Protein ID: WP_077266491.1
• Coordinates: 4617240..4617611 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: AXA51_RS22645
• Protein ID: WP_015569912.1
• Coordinates: 4616995..4617237 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA51_RS22615	4612054..4612854	+	801	WP_045142558.1	DeoR/GlpR family DNA-binding transcription regulator	-
AXA51_RS22620	4612973..4613572	+	600	WP_003861947.1	glucose-1-phosphatase	-
AXA51_RS22625	4613566..4614447	+	882	WP_003861949.1	virulence factor BrkB family protein	-
AXA51_RS22630	4614444..4614881	+	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AXA51_RS22635	4614926..4615867	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
AXA51_RS22640	4615876..4616796	-	921	WP_110292390.1	alpha/beta hydrolase	-
AXA51_RS22645	4616995..4617237	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
AXA51_RS22650	4617240..4617611	+	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
AXA51_RS22655	4617651..4618580	-	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
AXA51_RS22660	4618577..4619212	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
AXA51_RS22665	4619209..4620111	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T109454 WP_077266491.1 NZ_CP031571:4617240-4617611 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T109454 NZ_CP031571:4617240-4617611 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT109454 WP_015569912.1 NZ_CP031571:4616995-4617237 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT109454 NZ_CP031571:4616995-4617237 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATTGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAGCCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3