Detailed information of TA system TA109453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4235649..4236245
• Replicon: chromosome
• Accession: NZ_CP031571
• Organism: Enterobacter hormaechei strain N1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7G3EX93
• Locus tag: AXA51_RS20805
• Protein ID: WP_015570164.1
• Coordinates: 4235649..4235951 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A927HL08
• Locus tag: AXA51_RS20810
• Protein ID: WP_023295755.1
• Coordinates: 4235958..4236245 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA51_RS20785	4231868..4233217	+	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-
AXA51_RS20790	4233311..4234255	+	945	WP_015570166.1	ketopantoate/pantoate/pantothenate transporter PanS	-
AXA51_RS20795	4234306..4234578	+	273	WP_017382612.1	DUF3811 domain-containing protein	-
AXA51_RS20800	4234579..4235451	-	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
AXA51_RS20805	4235649..4235951	+	303	WP_015570164.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA51_RS20810	4235958..4236245	+	288	WP_023295755.1	putative addiction module antidote protein	Antitoxin
AXA51_RS20815	4236242..4237873	-	1632	WP_129541615.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11425.18 Da
• Isoelectric Point: 10.3736

>T109453 WP_015570164.1 NZ_CP031571:4235649-4235951 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGNSIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T109453 NZ_CP031571:4235649-4235951 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10101.59 Da
• Isoelectric Point: 5.7807

>AT109453 WP_023295755.1 NZ_CP031571:4235958-4236245 [Enterobacter hormaechei]
MHKLTPYDPANALLDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT109453 NZ_CP031571:4235958-4236245 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A7G3EX93