Detailed information of TA system TA109452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3921680..3922256
• Replicon: chromosome
• Accession: NZ_CP031571
• Organism: Enterobacter hormaechei strain N1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: AXA51_RS19300
• Protein ID: WP_015572580.1
• Coordinates: 3921969..3922256 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: AXA51_RS19295
• Protein ID: WP_017694570.1
• Coordinates: 3921680..3921982 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA51_RS19275	3917971..3918516	+	546	WP_006810254.1	porin	-
AXA51_RS19280	3918698..3920068	-	1371	WP_015572583.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
AXA51_RS19285	3920222..3920974	+	753	WP_039271800.1	AraC family transcriptional regulator	-
AXA51_RS19290	3921013..3921651	+	639	WP_017694569.1	LysE family translocator	-
AXA51_RS19295	3921680..3921982	-	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
AXA51_RS19300	3921969..3922256	-	288	WP_015572580.1	BrnT family toxin	Toxin
AXA51_RS19305	3922426..3923697	+	1272	WP_110292470.1	DUF445 domain-containing protein	-
AXA51_RS19310	3923694..3924770	-	1077	WP_015572578.1	DUF2955 domain-containing protein	-
AXA51_RS19315	3924760..3925827	-	1068	WP_026080654.1	HlyD family secretion protein	-
AXA51_RS19320	3925824..3926294	-	471	WP_015572576.1	MarR family transcriptional regulator	-
AXA51_RS19325	3926444..3927163	-	720	WP_110292471.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T109452 WP_015572580.1 NZ_CP031571:c3922256-3921969 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T109452 NZ_CP031571:c3922256-3921969 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAGTGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT109452 WP_017694570.1 NZ_CP031571:c3921982-3921680 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT109452 NZ_CP031571:c3921982-3921680 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGTGATCAGAACAAAAAATAA