Detailed information of TA system TA109437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 104782..105521
• Replicon: plasmid pIncFIB-1301491
• Accession: NZ_CP031569
• Organism: Enterobacter hormaechei strain 2013_1a

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A9E6WPH7
• Locus tag: AXA59_RS25750
• Protein ID: WP_013087269.1
• Coordinates: 104782..105267 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A9E6WPN8
• Locus tag: AXA59_RS25755
• Protein ID: WP_007869691.1
• Coordinates: 105255..105521 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA59_RS25730	100011..100999	-	989	Protein_112	tyrosine-type recombinase/integrase	-
AXA59_RS25735	101225..102565	+	1341	WP_049126869.1	hypothetical protein	-
AXA59_RS25740	103017..103952	-	936	WP_129557093.1	hypothetical protein	-
AXA59_RS25745	104023..104253	-	231	WP_047746533.1	hypothetical protein	-
AXA59_RS25750	104782..105267	-	486	WP_013087269.1	GNAT family N-acetyltransferase	Toxin
AXA59_RS25755	105255..105521	-	267	WP_007869691.1	DUF1778 domain-containing protein	Antitoxin
AXA59_RS25765	106173..107186	+	1014	WP_032670853.1	replication initiation protein	-
AXA59_RS25770	108076..108879	+	804	WP_032670854.1	DUF4225 domain-containing protein	-
AXA59_RS25775	108857..109180	-	324	WP_015572034.1	hypothetical protein	-
AXA59_RS25780	109195..109965	-	771	WP_032670855.1	conjugal transfer protein TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..140243	140243

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17736.45 Da
• Isoelectric Point: 9.5181

>T109437 WP_013087269.1 NZ_CP031569:c105267-104782 [Enterobacter hormaechei]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKRDTKQVVGFYSLATGSVNHVEAMGSLRRNM
PDPIPVIILARLAVDVSYRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T109437 NZ_CP031569:c105267-104782 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10080.46 Da
• Isoelectric Point: 4.3837

>AT109437 WP_007869691.1 NZ_CP031569:c105521-105255 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDEAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT109437 NZ_CP031569:c105521-105255 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA