Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 44764..45290 Replicon plasmid pSHV12-1301491
Accession NZ_CP031568
Organism Enterobacter hormaechei strain 2013_1a

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AXA59_RS24665 Protein ID WP_000323025.1
Coordinates 45003..45290 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AXA59_RS24660 Protein ID WP_000534858.1
Coordinates 44764..45003 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXA59_RS24640 41092..42330 + 1239 WP_000219087.1 IS110 family transposase -
AXA59_RS24645 42806..43378 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
AXA59_RS24650 43578..44501 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
AXA59_RS24660 44764..45003 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AXA59_RS24665 45003..45290 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AXA59_RS24670 45362..45520 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
AXA59_RS24675 46128..46448 - 321 WP_000332796.1 hypothetical protein -
AXA59_RS24680 46730..46933 - 204 WP_001015183.1 hypothetical protein -
AXA59_RS24685 46980..47330 - 351 WP_000743059.1 hypothetical protein -
AXA59_RS24690 47390..47992 - 603 WP_012695474.1 hypothetical protein -
AXA59_RS24695 48088..49032 - 945 WP_000778029.1 DUF5417 domain-containing protein -
AXA59_RS27235 49543..49719 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / catA2 / blaSHV-12 / sul2 / qacE / ere(A) / aac(6')-IIc / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / qnrB2 / aadA2 - 1..132677 132677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T109435 WP_000323025.1 NZ_CP031568:45003-45290 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T109435 NZ_CP031568:45003-45290 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT109435 WP_000534858.1 NZ_CP031568:44764-45003 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT109435 NZ_CP031568:44764-45003 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References