Detailed information of TA system TA109426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2153788..2154404
• Replicon: chromosome
• Accession: NZ_CP031565
• Organism: Enterobacter hormaechei strain 2013_1a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AXA59_RS10665
• Protein ID: WP_077266491.1
• Coordinates: 2154033..2154404 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: AXA59_RS10660
• Protein ID: WP_015569912.1
• Coordinates: 2153788..2154030 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA59_RS10630	2148847..2149647	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
AXA59_RS10635	2149766..2150365	+	600	WP_017694048.1	glucose-1-phosphatase	-
AXA59_RS10640	2150359..2151240	+	882	WP_003861949.1	virulence factor BrkB family protein	-
AXA59_RS10645	2151237..2151674	+	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AXA59_RS10650	2151719..2152660	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
AXA59_RS10655	2152669..2153589	-	921	WP_110871332.1	alpha/beta hydrolase	-
AXA59_RS10660	2153788..2154030	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
AXA59_RS10665	2154033..2154404	+	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
AXA59_RS10670	2154444..2155373	-	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
AXA59_RS10675	2155370..2156005	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
AXA59_RS10680	2156002..2156904	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T109426 WP_077266491.1 NZ_CP031565:2154033-2154404 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T109426 NZ_CP031565:2154033-2154404 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9408.66 Da
• Isoelectric Point: 4.4076

>AT109426 WP_015569912.1 NZ_CP031565:2153788-2154030 [Enterobacter hormaechei]
VSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT109426 NZ_CP031565:2153788-2154030 [Enterobacter hormaechei]
GTGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3