Detailed information of TA system TA109425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1454967..1455543
• Replicon: chromosome
• Accession: NZ_CP031565
• Organism: Enterobacter hormaechei strain 2013_1a

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: AXA59_RS07285
• Protein ID: WP_015572580.1
• Coordinates: 1455256..1455543 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: AXA59_RS07280
• Protein ID: WP_017694570.1
• Coordinates: 1454967..1455269 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA59_RS07260	1451263..1451808	+	546	WP_006810254.1	porin	-
AXA59_RS07265	1451985..1453355	-	1371	WP_110871120.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
AXA59_RS07270	1453509..1454261	+	753	WP_033486709.1	AraC family transcriptional regulator	-
AXA59_RS07275	1454300..1454938	+	639	WP_110871122.1	LysE family translocator	-
AXA59_RS07280	1454967..1455269	-	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
AXA59_RS07285	1455256..1455543	-	288	WP_015572580.1	BrnT family toxin	Toxin
AXA59_RS07290	1455713..1456984	+	1272	WP_110871124.1	DUF445 domain-containing protein	-
AXA59_RS07295	1456981..1458057	-	1077	WP_015572578.1	DUF2955 domain-containing protein	-
AXA59_RS07300	1458047..1459114	-	1068	WP_108394134.1	HlyD family secretion protein	-
AXA59_RS07305	1459111..1459581	-	471	WP_023295868.1	MarR family transcriptional regulator	-
AXA59_RS07310	1459731..1460450	-	720	WP_045330246.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T109425 WP_015572580.1 NZ_CP031565:c1455543-1455256 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T109425 NZ_CP031565:c1455543-1455256 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGTGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT109425 WP_017694570.1 NZ_CP031565:c1455269-1454967 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT109425 NZ_CP031565:c1455269-1454967 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA