Detailed information of TA system TA109422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 218342..218952
• Replicon: chromosome
• Accession: NZ_CP031565
• Organism: Enterobacter hormaechei strain 2013_1a

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: AXA59_RS01270
• Protein ID: WP_012906750.1
• Coordinates: 218342..218521 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A6G3F240
• Locus tag: AXA59_RS01275
• Protein ID: WP_045339414.1
• Coordinates: 218542..218952 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA59_RS01225	213377..213631	-	255	WP_045341642.1	DUF333 domain-containing protein	-
AXA59_RS01230	213943..214971	+	1029	WP_045344202.1	sensor domain-containing diguanylate cyclase	-
AXA59_RS01235	215017..215115	+	99	WP_015570793.1	YoaK family small membrane protein	-
AXA59_RS01240	215116..215190	+	75	WP_015570794.1	protein YoaJ	-
AXA59_RS01245	215244..215492	-	249	WP_003857896.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
AXA59_RS01250	215862..215954	+	93	WP_071524166.1	stress response membrane protein YncL	-
AXA59_RS01255	216079..217578	+	1500	WP_110870786.1	cyclic diguanylate phosphodiesterase	-
AXA59_RS01260	217583..217828	-	246	WP_022650806.1	YmjA family protein	-
AXA59_RS01265	217904..218071	-	168	WP_104010168.1	isocitrate dehydrogenase	-
AXA59_RS01270	218342..218521	+	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
AXA59_RS01275	218542..218952	+	411	WP_045339414.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AXA59_RS01280	219034..219600	+	567	WP_047353913.1	serine acetyltransferase	-
AXA59_RS01285	219604..220026	-	423	WP_064489211.1	tail fiber assembly protein	-
AXA59_RS27145	221912..222589	-	678	Protein_253	phage tail protein	-
AXA59_RS01295	222592..223143	-	552	WP_045345258.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	215017..259260	44243

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T109422 WP_012906750.1 NZ_CP031565:218342-218521 [Enterobacter hormaechei]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T109422 NZ_CP031565:218342-218521 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTGAAAGGCAGCCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14890.79 Da
• Isoelectric Point: 4.3170

>AT109422 WP_045339414.1 NZ_CP031565:218542-218952 [Enterobacter hormaechei]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Nucleotide

• Length: 411 bp

>AT109422 NZ_CP031565:218542-218952 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATTGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCAGAAGCAACGACCGTCGAAAACTGGCTTTCTGATCCGGAGTATGCCGGTGCTGTATGGGCCCTGGTTGAC
GTGGATATAACTCGTCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTTCCTTCACTGCTGATCCGACGTATCGATCA
GTTTGTGGCCGCGCATCCTGAATATGGCAGCCGGTCTGGCTTCCTGTCTCGTGTCGCGGCAGATAAAGTAATTGGACGAG
AAAAACGATAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4

Antitoxin

• AlphaFold DB: A0A6G3F240