Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 565756..566280 Replicon chromosome
Accession NZ_CP031561
Organism Staphylococcus pseudintermedius strain AP20

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag DNI88_RS02625 Protein ID WP_014613379.1
Coordinates 565927..566280 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag DNI88_RS02620 Protein ID WP_014613378.1
Coordinates 565756..565926 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNI88_RS02595 (DNI88_02605) 561645..562118 + 474 WP_014613373.1 PH domain-containing protein -
DNI88_RS02600 (DNI88_02610) 562111..563640 + 1530 WP_105503167.1 PH domain-containing protein -
DNI88_RS02605 (DNI88_02615) 563624..564142 + 519 WP_014613375.1 PH domain-containing protein -
DNI88_RS02610 (DNI88_02620) 564139..564489 + 351 WP_014613376.1 holo-ACP synthase -
DNI88_RS02615 (DNI88_02625) 564523..565671 + 1149 WP_065354670.1 alanine racemase -
DNI88_RS02620 (DNI88_02630) 565756..565926 + 171 WP_014613378.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
DNI88_RS02625 (DNI88_02635) 565927..566280 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DNI88_RS02630 (DNI88_02640) 566338..567342 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
DNI88_RS02635 (DNI88_02645) 567421..567747 + 327 WP_014613381.1 anti-sigma factor antagonist -
DNI88_RS02640 (DNI88_02650) 567747..568226 + 480 WP_031253870.1 anti-sigma B factor RsbW -
DNI88_RS02645 (DNI88_02655) 568201..568971 + 771 WP_105503168.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T109420 WP_014613379.1 NZ_CP031561:565927-566280 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T109420 NZ_CP031561:565927-566280 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT109420 WP_014613378.1 NZ_CP031561:565756-565926 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT109420 NZ_CP031561:565756-565926 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References