Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 550689..551210 Replicon chromosome
Accession NZ_CP031561
Organism Staphylococcus pseudintermedius strain AP20

Toxin (Protein)


Gene name TsdT Uniprot ID A0A3D8YLE8
Locus tag DNI88_RS02550 Protein ID WP_014613364.1
Coordinates 550989..551210 (+) Length 74 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A166R1D0
Locus tag DNI88_RS02545 Protein ID WP_014613363.1
Coordinates 550689..550976 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNI88_RS02520 (DNI88_02530) 545950..546753 + 804 WP_105503166.1 hydroxyethylthiazole kinase -
DNI88_RS02525 (DNI88_02535) 546743..547381 + 639 WP_014613359.1 thiamine phosphate synthase -
DNI88_RS02530 (DNI88_02540) 547470..548336 + 867 WP_014613360.1 membrane protein insertase YidC -
DNI88_RS02535 (DNI88_02545) 548390..549046 - 657 WP_014613361.1 HD domain-containing protein -
DNI88_RS02540 (DNI88_02550) 549067..550557 - 1491 WP_065520420.1 cardiolipin synthase -
DNI88_RS02545 (DNI88_02555) 550689..550976 + 288 WP_014613363.1 copper-sensing transcriptional repressor CsoR Antitoxin
DNI88_RS02550 (DNI88_02560) 550989..551210 + 222 WP_014613364.1 heavy metal-associated domain-containing protein Toxin
DNI88_RS02555 (DNI88_02565) 551214..551420 + 207 WP_014613365.1 cation transporter -
DNI88_RS02560 (DNI88_02570) 551521..553701 + 2181 WP_096636894.1 heavy metal translocating P-type ATPase -
DNI88_RS02565 553787..553933 + 147 WP_014613367.1 Lmo0850 family protein -
DNI88_RS02570 (DNI88_02580) 555052..555780 + 729 WP_014613368.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(21-64)

Antitoxin

Metal-sensitive transcriptional repressor

(3-93)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8065.10 Da        Isoelectric Point: 4.3480

>T109419 WP_014613364.1 NZ_CP031561:550989-551210 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGFPVIQSHKGV

Download         Length: 222 bp

>T109419 NZ_CP031561:550989-551210 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10997.59 Da        Isoelectric Point: 7.0504

>AT109419 WP_014613363.1 NZ_CP031561:550689-550976 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Download         Length: 288 bp

>AT109419 NZ_CP031561:550689-550976 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3D8YLE8

Antitoxin

Source ID Structure
AlphaFold DB A0A166R1D0

References