Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 473862..474604 Replicon chromosome
Accession NZ_CP031560
Organism Dickeya dianthicola strain ME23

Toxin (Protein)


Gene name ataT Uniprot ID E0SIC1
Locus tag DZA65_RS02165 Protein ID WP_013316117.1
Coordinates 473862..474341 (-) Length 160 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A3A4C8A5
Locus tag DZA65_RS02170 Protein ID WP_024104363.1
Coordinates 474332..474604 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DZA65_RS02135 469082..469405 + 324 WP_024104357.1 DNA-binding transcriptional regulator -
DZA65_RS02140 469692..471419 - 1728 WP_103415798.1 retron Ec67 family RNA-directed DNA polymerase/endonuclease -
DZA65_RS02145 472540..472860 + 321 WP_024104360.1 type II toxin-antitoxin system RelE/ParE family toxin -
DZA65_RS02150 472850..473134 + 285 WP_024104361.1 helix-turn-helix domain-containing protein -
DZA65_RS02160 473432..473833 - 402 WP_024104362.1 hypothetical protein -
DZA65_RS02165 473862..474341 - 480 WP_013316117.1 GNAT family N-acetyltransferase Toxin
DZA65_RS02170 474332..474604 - 273 WP_024104363.1 DUF1778 domain-containing protein Antitoxin
DZA65_RS02175 475094..475517 + 424 Protein_416 DUF4102 domain-containing protein -
DZA65_RS02185 476425..476646 - 222 WP_024108131.1 DUF1778 domain-containing protein -
DZA65_RS02195 476944..478332 + 1389 WP_024104366.1 hypothetical protein -
DZA65_RS02200 478482..478820 - 339 WP_024104367.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 461490..480096 18606
- flank IS/Tn - - 475948..476262 314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(28-147)

Antitoxin

Vibrio phage ICP1, Orf50

(10-85)


Sequences

Toxin        


Download         Length: 160 a.a.        Molecular weight: 17564.34 Da        Isoelectric Point: 9.5367

>T109401 WP_013316117.1 NZ_CP031560:c474341-473862 [Dickeya dianthicola]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVPSEIQEHTLLLSLW

Download         Length: 480 bp

>T109401 NZ_CP031560:c474341-473862 [Dickeya dianthicola]
GTGGGAATAACGGCACCTGAATTATTGCTGCCTCAACATGCGGTTGTGGATTTTCATTGTTCAGAGCCGTCATTGAATGA
ATGGCTGAAACGCAAGGCGTTAAAAAATCAGACGCTGGGCGCTTCACGCACCTTCGTGGTGTGTGAAGCCGGTACACAGC
GTGTGGTGGGGTTCTATGCCCTCGCGTCGGGCAGCATCCAGCGTCAGGTTGCGCCGGGCGCATTCCGGCGCAATATGCCT
GACCCCATTCCTGTTCTGGTGCTCGGTAGATTAGCCGTTGATGAACGTTATCAACGTATGGGGATCGGTGCCGGGCTCCT
GAAAGATGCGGTACTCCGCTCCCGCAATGTAGCGCAGCAGGTAGGCAACAAAGCGTTATTGGTACATGCGTTATCTGATG
AGGCAAAAGCGTTCTACCAATACTGGGGTTTTGTCCCGTCAGAAATACAGGAACACACCTTATTATTATCGTTATGGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10390.84 Da        Isoelectric Point: 4.6702

>AT109401 WP_024104363.1 NZ_CP031560:c474604-474332 [Dickeya dianthicola]
MRTETKETPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRMN
ALLNRKSPWE

Download         Length: 273 bp

>AT109401 NZ_CP031560:c474604-474332 [Dickeya dianthicola]
ATGCGAACAGAGACAAAAGAAACGCCGATTAATATTCGTGCTAAAGCATCACAGCGGGAGCTGATTGATGTCGCCGCTAA
ATTGCTGTCGAAATCAAGAACAGAGTTTATCCTTGATGCTGCCTGCCGTGAGGCTGAGGATGTGTTGTTGGATCAAAGGC
TGTTTCTGGTCAATGATGAACAATATGATGCTTTCATTCAGGTGCTGGAATCACCTGTCACAGATAATCCGCGTATGAAT
GCATTACTGAACAGGAAATCTCCGTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3N0G158

Antitoxin

Source ID Structure
AlphaFold DB A0A3A4C8A5

References