Detailed information of TA system TA109396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3073623..3074228
• Replicon: chromosome
• Accession: NZ_CP031555
• Organism: Thalassospira indica strain PB8BT (=MCCC 1A01103T=LMG 29620T)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: DY252_RS14540
• Protein ID: WP_082923557.1
• Coordinates: 3073623..3073811 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A199YG30
• Locus tag: DY252_RS14545
• Protein ID: WP_064789799.1
• Coordinates: 3073860..3074228 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DY252_RS14520	3069552..3070460	+	909	WP_064789843.1	DMT family transporter	-
DY252_RS14525	3070603..3071628	+	1026	WP_174713882.1	DMT family transporter	-
DY252_RS14530	3071643..3072101	-	459	WP_082923558.1	GNAT family N-acetyltransferase	-
DY252_RS14535	3072098..3073126	-	1029	WP_129542808.1	sensor domain-containing diguanylate cyclase	-
DY252_RS22275	3073013..3073417	-	405	Protein_2855	PAS domain-containing protein	-
DY252_RS14540	3073623..3073811	+	189	WP_082923557.1	type II toxin-antitoxin system HicA family toxin	Toxin
DY252_RS14545	3073860..3074228	+	369	WP_064789799.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DY252_RS14550	3074269..3074850	-	582	WP_129542742.1	hypothetical protein	-
DY252_RS14555	3075768..3076745	+	978	WP_082923555.1	MBL fold metallo-hydrolase	-
DY252_RS14560	3076849..3077475	+	627	WP_064789796.1	hypothetical protein	-
DY252_RS14570	3077875..3079020	-	1146	WP_064789795.1	tRNA 2-thiouridine(34) synthase MnmA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (8-114)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7103.27 Da
• Isoelectric Point: 11.1981

>T109396 WP_082923557.1 NZ_CP031555:3073623-3073811 [Thalassospira indica]
MDSREILKRLKDDGWEIVRTKGSHHQLTHPTKPGRVTVPHPKRDIPKGTVKSIERQSGIKLL

Nucleotide

• Length: 189 bp

>T109396 NZ_CP031555:3073623-3073811 [Thalassospira indica]
ATGGATAGCAGGGAAATACTCAAACGGCTGAAAGATGATGGCTGGGAAATCGTTCGCACGAAAGGCAGTCACCATCAGCT
AACGCACCCGACAAAACCGGGGCGCGTGACAGTGCCACACCCCAAGCGGGATATCCCAAAGGGCACAGTAAAAAGCATTG
AAAGGCAATCGGGTATAAAGCTGCTGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13089.81 Da
• Isoelectric Point: 4.2870

>AT109396 WP_064789799.1 NZ_CP031555:3073860-3074228 [Thalassospira indica]
MKQTYWGLVHTDDDSGFGISFPDFPGCVSAADTMTELVELGTEALNFHIEGMHADGEDIPTPSPVVELEDGAVGIVAITA
TIPGKKRRINLTLDANLIDLIEAKYGKRAVSSFLEEAGRKAL

Nucleotide

• Length: 369 bp

>AT109396 NZ_CP031555:3073860-3074228 [Thalassospira indica]
ATGAAACAGACCTATTGGGGTTTGGTCCACACTGACGATGATAGCGGCTTCGGGATTTCCTTTCCGGATTTCCCTGGTTG
CGTCTCTGCTGCTGATACAATGACGGAATTGGTGGAGTTGGGCACAGAGGCCCTTAACTTCCACATCGAAGGCATGCATG
CCGATGGCGAAGACATCCCGACCCCCTCGCCGGTTGTCGAGCTTGAAGACGGTGCCGTCGGTATTGTCGCCATTACCGCG
ACCATTCCGGGGAAAAAGCGCCGGATCAACCTGACCCTTGATGCGAACCTGATTGATTTGATTGAAGCGAAATACGGCAA
GCGCGCGGTTTCGAGCTTCCTTGAGGAGGCTGGGCGGAAGGCGCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A199YG30