Detailed information of TA system TA109388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 41406..41928
• Replicon: plasmid unnamed4
• Accession: NZ_CP031550
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: DS732_RS31265
• Protein ID: WP_000638823.1
• Coordinates: 41647..41928 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: DS732_RS31260
• Protein ID: WP_000121741.1
• Coordinates: 41406..41657 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS31210	36492..36935	+	444	WP_000964330.1	NfeD family protein	-
DS732_RS31895	36939..37109	+	171	WP_000550720.1	hypothetical protein	-
DS732_RS31215	37120..37566	+	447	WP_001243165.1	hypothetical protein	-
DS732_RS31220	37599..37856	+	258	WP_001542015.1	hypothetical protein	-
DS732_RS31230	38137..38394	+	258	WP_000739144.1	hypothetical protein	-
DS732_RS31235	38398..39393	+	996	WP_001028543.1	type IV secretion system protein	-
DS732_RS31240	39399..40040	+	642	WP_001425343.1	conjugal transfer protein TrbJ	-
DS732_RS31245	40042..40296	+	255	WP_001043555.1	EexN family lipoprotein	-
DS732_RS31250	40309..40596	+	288	WP_001032611.1	EexN family lipoprotein	-
DS732_RS31260	41406..41657	+	252	WP_000121741.1	hypothetical protein	Antitoxin
DS732_RS31265	41647..41928	+	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DS732_RS31270	42017..42297	+	281	Protein_55	conjugal transfer protein	-
DS732_RS31275	42300..43536	+	1237	Protein_56	TcpQ domain-containing protein	-
DS732_RS31280	43542..43982	+	441	Protein_57	type IV pilus biogenesis protein PilM	-
DS732_RS31285	44101..44499	+	399	WP_001153665.1	hypothetical protein	-
DS732_RS31290	44520..45104	+	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
DS732_RS31295	45206..45394	+	189	WP_050567133.1	hypothetical protein	-
DS732_RS31300	45465..45785	+	321	WP_000362080.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1 / blaCTX-M-55	-	1..66945	66945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T109388 WP_000638823.1 NZ_CP031550:41647-41928 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T109388 NZ_CP031550:41647-41928 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT109388 WP_000121741.1 NZ_CP031550:41406-41657 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT109388 NZ_CP031550:41406-41657 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1