Detailed information of TA system TA109387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 26547..27041
• Replicon: plasmid unnamed4
• Accession: NZ_CP031550
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: -
• Locus tag: DS732_RS31140
• Protein ID: WP_116794661.1
• Coordinates: 26793..27041 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: DS732_RS31135
• Protein ID: WP_000151456.1
• Coordinates: 26547..26789 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS31100	21649..21972	-	324	WP_000427403.1	hypothetical protein	-
DS732_RS31110	22553..22882	-	330	WP_000855120.1	hypothetical protein	-
DS732_RS31120	23641..24903	+	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
DS732_RS31125	25156..26031	+	876	WP_015387340.1	class A extended-spectrum beta-lactamase CTX-M-55	-
DS732_RS31130	26078..26410	-	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-
DS732_RS31135	26547..26789	-	243	WP_000151456.1	hypothetical protein	Antitoxin
DS732_RS31140	26793..27041	-	249	WP_116794661.1	hypothetical protein	Toxin
DS732_RS31145	27038..27580	-	543	WP_001083904.1	hypothetical protein	-
DS732_RS31150	27684..28199	-	516	WP_001025393.1	J domain-containing protein	-
DS732_RS31155	28789..29454	+	666	WP_000532148.1	P-loop NTPase	-
DS732_RS32040	29528..29812	+	285	WP_001162371.1	hypothetical protein	-
DS732_RS31165	29830..30426	-	597	WP_000125165.1	hypothetical protein	-
DS732_RS31170	30576..30737	-	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
DS732_RS31885	30924..31085	-	162	WP_001128311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1 / blaCTX-M-55	-	1..66945	66945
	-	flank	IS/Tn	blaCTX-M-55	-	23641..26031	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9726.28 Da
• Isoelectric Point: 10.2462

>T109387 WP_116794661.1 NZ_CP031550:c27041-26793 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
GE

Nucleotide

• Length: 249 bp

>T109387 NZ_CP031550:c27041-26793 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GGAGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT109387 WP_000151456.1 NZ_CP031550:c26789-26547 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT109387 NZ_CP031550:c26789-26547 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A3G9GNF5