Detailed information of TA system TA109386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 20524..21025
• Replicon: plasmid unnamed4
• Accession: NZ_CP031550
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: DS732_RS31085
• Protein ID: WP_000702247.1
• Coordinates: 20765..21025 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: DS732_RS31080
• Protein ID: WP_001132407.1
• Coordinates: 20524..20775 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS31030	15581..16333	+	753	WP_000797966.1	conjugal transfer protein TraL	-
DS732_RS31035	16335..17042	+	708	WP_021503228.1	hypothetical protein	-
DS732_RS31040	17062..17388	+	327	WP_063078684.1	hypothetical protein	-
DS732_RS31045	17519..17956	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
DS732_RS31050	17981..18163	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
DS732_RS31055	18721..18903	+	183	WP_001481829.1	hypothetical protein	-
DS732_RS31060	19010..19276	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
DS732_RS31065	19696..19974	-	279	WP_000545938.1	hypothetical protein	-
DS732_RS31080	20524..20775	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
DS732_RS31085	20765..21025	-	261	WP_000702247.1	hypothetical protein	Toxin
DS732_RS31090	21077..21301	-	225	WP_000814381.1	hypothetical protein	-
DS732_RS31095	21398..21607	-	210	WP_000866036.1	hypothetical protein	-
DS732_RS31100	21649..21972	-	324	WP_000427403.1	hypothetical protein	-
DS732_RS31110	22553..22882	-	330	WP_000855120.1	hypothetical protein	-
DS732_RS31120	23641..24903	+	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1 / blaCTX-M-55	-	1..66945	66945
	-	flank	IS/Tn	blaCTX-M-55	-	23641..26031	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T109386 WP_000702247.1 NZ_CP031550:c21025-20765 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T109386 NZ_CP031550:c21025-20765 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT109386 WP_001132407.1 NZ_CP031550:c20775-20524 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT109386 NZ_CP031550:c20775-20524 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0