Detailed information of TA system TA109384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 106048..106691
• Replicon: plasmid unnamed3
• Accession: NZ_CP031549
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: DS732_RS30420
• Protein ID: WP_001044768.1
• Coordinates: 106275..106691 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: DS732_RS30415
• Protein ID: WP_001261287.1
• Coordinates: 106048..106278 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS30375	101333..103027	-	1695	WP_000105636.1	mercury(II) reductase	-
DS732_RS30380	103079..103501	-	423	WP_001340589.1	organomercurial transporter MerC	-
DS732_RS30385	103537..103812	-	276	WP_000732292.1	mercury resistance system periplasmic binding protein MerP	-
DS732_RS30390	103826..104176	-	351	WP_001294663.1	mercuric transport protein MerT	-
DS732_RS30395	104248..104682	+	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
DS732_RS30400	104729..105433	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
DS732_RS30405	105480..105752	-	273	WP_061602529.1	hypothetical protein	-
DS732_RS30415	106048..106278	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DS732_RS30420	106275..106691	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
DS732_RS30425	106853..108991	-	2139	WP_000350638.1	AAA family ATPase	-
DS732_RS30430	109345..109602	+	258	WP_000343085.1	hypothetical protein	-
DS732_RS30435	109602..110192	+	591	WP_000194575.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / erm(B) / mph(A) / floR	-	1..189020	189020
	-	flank	IS/Tn	-	-	104729..105433	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T109384 WP_001044768.1 NZ_CP031549:106275-106691 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T109384 NZ_CP031549:106275-106691 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT109384 WP_001261287.1 NZ_CP031549:106048-106278 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT109384 NZ_CP031549:106048-106278 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4