Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 151583..152118 Replicon plasmid unnamed2
Accession NZ_CP031548
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag DS732_RS29285 Protein ID WP_001270417.1
Coordinates 151583..151870 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag DS732_RS29290 Protein ID WP_001132895.1
Coordinates 151867..152118 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS29240 147128..147237 + 110 Protein_169 IS5/IS1182 family transposase -
DS732_RS29245 147760..148010 + 251 Protein_170 type II toxin-antitoxin system ParD family antitoxin -
DS732_RS29265 148654..149394 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -
DS732_RS29270 149679..150656 - 978 WP_000361610.1 RepB family plasmid replication initiator protein -
DS732_RS29280 151336..151584 - 249 Protein_173 integrase core domain-containing protein -
DS732_RS29285 151583..151870 - 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DS732_RS29290 151867..152118 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DS732_RS29295 152291..152503 - 213 WP_000402944.1 hypothetical protein -
DS732_RS29305 152877..154082 + 1206 WP_000817634.1 AAA family ATPase -
DS732_RS29310 154079..155041 + 963 WP_000756327.1 ParB/RepB/Spo0J family partition protein -
DS732_RS29315 155275..156052 + 778 Protein_179 hypothetical protein -
DS732_RS29325 156219..156362 + 144 WP_000270807.1 DUF1281 domain-containing protein -
DS732_RS29330 156369..156728 + 360 WP_001385002.1 DUF1281 domain-containing protein -
DS732_RS29340 156792..157103 + 312 WP_001309256.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyC / hlyA / hlyB / hlyD / eltB / eltA 1..221041 221041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T109379 WP_001270417.1 NZ_CP031548:c151870-151583 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T109379 NZ_CP031548:c151870-151583 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT109379 WP_001132895.1 NZ_CP031548:c152118-151867 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT109379 NZ_CP031548:c152118-151867 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References