Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 130378..130925 Replicon plasmid unnamed2
Accession NZ_CP031548
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag DS732_RS29125 Protein ID WP_000969996.1
Coordinates 130644..130925 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A2I6FMZ7
Locus tag DS732_RS29120 Protein ID WP_000079930.1
Coordinates 130378..130647 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 126096..126157 - 62 NuclAT_2 - -
- 126096..126157 - 62 NuclAT_2 - -
- 126096..126157 - 62 NuclAT_2 - -
- 126096..126157 - 62 NuclAT_2 - -
DS732_RS29090 127462..127602 + 141 WP_064717426.1 type I toxin-antitoxin system Hok family toxin -
DS732_RS29095 127882..128139 + 258 WP_000083834.1 replication regulatory protein RepA -
DS732_RS29105 128373..128447 + 75 WP_032319616.1 RepA leader peptide Tap -
DS732_RS29110 128440..129462 + 1023 WP_000840938.1 replication initiation protein -
DS732_RS29120 130378..130647 + 270 WP_000079930.1 hypothetical protein Antitoxin
DS732_RS29125 130644..130925 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DS732_RS31785 130971..131700 + 730 Protein_151 3'-5' exonuclease -
DS732_RS29140 131770..132015 - 246 Protein_152 DDE-type integrase/transposase/recombinase -
DS732_RS29145 132032..133108 - 1077 WP_039022834.1 IS110 family transposase -
DS732_RS29155 133390..134249 - 860 Protein_154 IS3 family transposase -
DS732_RS29165 134363..134759 - 397 Protein_155 ATP-binding protein -
DS732_RS29170 135107..135481 - 375 WP_015675362.1 heat-labile enterotoxin LT subunit B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyC / hlyA / hlyB / hlyD / eltB / eltA 1..221041 221041
- inside IScluster/Tn - eltB / eltA 116914..144870 27956


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T109378 WP_000969996.1 NZ_CP031548:130644-130925 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T109378 NZ_CP031548:130644-130925 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2055

>AT109378 WP_000079930.1 NZ_CP031548:130378-130647 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVADK

Download         Length: 270 bp

>AT109378 NZ_CP031548:130378-130647 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCTGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A2I6FMZ7

References