Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 79204..79763 Replicon plasmid unnamed2
Accession NZ_CP031548
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag DS732_RS28760 Protein ID WP_000421257.1
Coordinates 79204..79479 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag DS732_RS28765 Protein ID WP_001178089.1
Coordinates 79479..79763 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS28735 75540..75905 + 366 WP_000124098.1 hypothetical protein -
DS732_RS28740 75905..77092 + 1188 WP_000937614.1 IS91-like element IS91 family transposase -
DS732_RS28750 77369..77734 + 366 WP_000091308.1 hypothetical protein -
DS732_RS28755 77734..78921 + 1188 WP_000937591.1 IS91 family transposase -
DS732_RS28760 79204..79479 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DS732_RS28765 79479..79763 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
DS732_RS28775 80673..81530 - 858 WP_024233440.1 incFII family plasmid replication initiator RepA -
DS732_RS28780 81523..81597 - 75 WP_001365705.1 RepA leader peptide Tap -
DS732_RS28790 81834..82088 - 255 WP_000083830.1 replication regulatory protein RepA -
DS732_RS28795 82353..82583 - 231 WP_001736714.1 hypothetical protein -
DS732_RS28800 82747..83346 - 600 WP_063082122.1 hypothetical protein -
DS732_RS28805 83733..83933 - 201 WP_015059022.1 hypothetical protein -
DS732_RS28810 84065..84625 - 561 WP_000139328.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyC / hlyA / hlyB / hlyD / eltB / eltA 1..221041 221041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T109377 WP_000421257.1 NZ_CP031548:c79479-79204 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T109377 NZ_CP031548:c79479-79204 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10456.86 Da        Isoelectric Point: 5.6558

>AT109377 WP_001178089.1 NZ_CP031548:c79763-79479 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT109377 NZ_CP031548:c79763-79479 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGTCTCAGTACTGACAATCCGTTACCGGTGCCCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References