Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 61900..62422 Replicon plasmid unnamed2
Accession NZ_CP031548
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag DS732_RS28640 Protein ID WP_000220560.1
Coordinates 62141..62422 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag DS732_RS28635 Protein ID WP_000121743.1
Coordinates 61900..62151 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS28610 57797..58162 + 366 WP_000091308.1 hypothetical protein -
DS732_RS28615 58162..59349 + 1188 WP_000937591.1 IS91 family transposase -
DS732_RS28620 59539..59790 - 252 Protein_66 hypothetical protein -
DS732_RS28625 59830..60666 - 837 WP_001050931.1 replication initiation protein -
DS732_RS28635 61900..62151 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
DS732_RS28640 62141..62422 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DS732_RS28645 62568..62891 + 324 WP_001181907.1 hypothetical protein -
DS732_RS28650 62936..63181 + 246 WP_000356545.1 hypothetical protein -
DS732_RS28655 63171..63386 + 216 WP_001180116.1 hypothetical protein -
DS732_RS28660 63775..64962 - 1188 WP_000937591.1 IS91 family transposase -
DS732_RS28665 64962..65327 - 366 WP_000091308.1 hypothetical protein -
DS732_RS28675 65604..66791 - 1188 WP_116794643.1 IS91-like element IS91 family transposase -
DS732_RS28680 66791..67156 - 366 WP_000124098.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyC / hlyA / hlyB / hlyD / eltB / eltA 1..221041 221041
- inside IScluster/Tn - - 54459..68666 14207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T109376 WP_000220560.1 NZ_CP031548:62141-62422 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T109376 NZ_CP031548:62141-62422 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT109376 WP_000121743.1 NZ_CP031548:61900-62151 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT109376 NZ_CP031548:61900-62151 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References