Detailed information of TA system TA109373

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5135736..5136571
• Replicon: chromosome
• Accession: NZ_CP031546
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I6F8K9
• Locus tag: DS732_RS26625
• Protein ID: WP_063102707.1
• Coordinates: 5136194..5136571 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2I6F7Z1
• Locus tag: DS732_RS26620
• Protein ID: WP_063102708.1
• Coordinates: 5135736..5136104 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS26580	5131397..5132281	+	885	WP_063102632.1	50S ribosome-binding GTPase	-
DS732_RS26585	5132400..5133077	+	678	WP_063116756.1	hypothetical protein	-
DS732_RS26590	5133083..5133316	+	234	WP_063102713.1	DUF905 family protein	-
DS732_RS26595	5133406..5134224	+	819	WP_063102712.1	DUF945 domain-containing protein	-
DS732_RS26600	5134315..5134797	+	483	WP_063102711.1	antirestriction protein	-
DS732_RS26610	5134813..5135289	+	477	WP_063116755.1	RadC family protein	-
DS732_RS26615	5135352..5135573	+	222	WP_063102709.1	DUF987 domain-containing protein	-
DS732_RS26620	5135736..5136104	+	369	WP_063102708.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DS732_RS26625	5136194..5136571	+	378	WP_063102707.1	TA system toxin CbtA family protein	Toxin
DS732_RS26630	5136568..5137056	+	489	WP_063102706.1	hypothetical protein	-
DS732_RS26635	5137068..5137265	+	198	WP_063102705.1	DUF957 domain-containing protein	-
DS732_RS26640	5137350..5138192	+	843	WP_063102704.1	DUF4942 domain-containing protein	-
DS732_RS26655	5138988..5140172	+	1185	WP_001172893.1	sugar efflux transporter	-
DS732_RS26660	5140283..5141206	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14077.05 Da
• Isoelectric Point: 7.9086

>T109373 WP_063102707.1 NZ_CP031546:5136194-5136571 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRNNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T109373 NZ_CP031546:5136194-5136571 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAA
CAACTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.26 Da
• Isoelectric Point: 6.3147

>AT109373 WP_063102708.1 NZ_CP031546:5135736-5136104 [Escherichia coli]
VSDTFSGTTHPDDNNSRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT109373 NZ_CP031546:5135736-5136104 [Escherichia coli]
GTGTCAGACACATTCTCCGGAACAACTCATCCCGATGATAACAACAGCCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTACATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2I6F8K9

Antitoxin

• AlphaFold DB: A0A2I6F7Z1