Detailed information of TA system TA109369

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4314635..4315467
• Replicon: chromosome
• Accession: NZ_CP031546
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A346GNK2
• Locus tag: DS732_RS22275
• Protein ID: WP_032082789.1
• Coordinates: 4315093..4315467 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M2U6P5
• Locus tag: DS732_RS22270
• Protein ID: WP_001774606.1
• Coordinates: 4314635..4315003 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS22225	4310495..4311172	+	678	WP_001097305.1	hypothetical protein	-
DS732_RS22230	4311178..4311411	+	234	WP_001278287.1	DUF905 family protein	-
DS732_RS22240	4311510..4312328	+	819	WP_116794640.1	DUF945 domain-containing protein	-
DS732_RS22245	4312328..4312573	+	246	WP_032082791.1	hypothetical protein	-
DS732_RS22250	4312667..4313146	+	480	WP_032082790.1	antirestriction protein	-
DS732_RS22255	4313162..4313638	+	477	WP_001297237.1	RadC family protein	-
DS732_RS22260	4313701..4313922	+	222	WP_063102745.1	DUF987 domain-containing protein	-
DS732_RS22265	4313941..4314585	+	645	WP_001774605.1	hypothetical protein	-
DS732_RS22270	4314635..4315003	+	369	WP_001774606.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
DS732_RS22275	4315093..4315467	+	375	WP_032082789.1	TA system toxin CbtA family protein	Toxin
DS732_RS22280	4315464..4315952	+	489	WP_032082788.1	hypothetical protein	-
DS732_RS22285	4315969..4316166	+	198	WP_032082787.1	DUF957 domain-containing protein	-
DS732_RS22290	4316263..4317108	+	846	WP_032082786.1	DUF4942 domain-containing protein	-
DS732_RS22295	4317179..4318714	+	1536	WP_001609560.1	EAL domain-containing protein	-
DS732_RS22300	4319050..4319586	-	537	WP_000942786.1	GspM family type II secretion system protein YghD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14214.29 Da
• Isoelectric Point: 8.2905

>T109369 WP_032082789.1 NZ_CP031546:4315093-4315467 [Escherichia coli]
MKTLPDTHVREASRCPPPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRTTGLMTRDNYRTVNNITLSKHPEKR

Nucleotide

• Length: 375 bp

>T109369 NZ_CP031546:4315093-4315467 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGCCTCCCGTCACCATCTGGCAGATACTGTTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGACGACCGGCCTGATGACCCGCGA
CAATTACAGAACAGTAAATAACATTACCCTGAGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.31 Da
• Isoelectric Point: 5.8746

>AT109369 WP_001774606.1 NZ_CP031546:4314635-4315003 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT109369 NZ_CP031546:4314635-4315003 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A346GNK2

Antitoxin

• AlphaFold DB: A0A1M2U6P5