Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4180544..4181198 Replicon chromosome
Accession NZ_CP031546
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag DS732_RS21515 Protein ID WP_000244777.1
Coordinates 4180544..4180951 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DS732_RS21520 Protein ID WP_000354046.1
Coordinates 4180932..4181198 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS21495 4176501..4178234 - 1734 WP_063103078.1 single-stranded-DNA-specific exonuclease RecJ -
DS732_RS21500 4178240..4178950 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DS732_RS21505 4178975..4179871 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DS732_RS21510 4179983..4180504 + 522 WP_001055874.1 flavodoxin FldB -
DS732_RS21515 4180544..4180951 - 408 WP_000244777.1 toxin CptA Toxin
DS732_RS21520 4180932..4181198 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DS732_RS21525 4181441..4182421 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
DS732_RS21530 4182498..4183157 - 660 WP_000250274.1 hemolysin III family protein -
DS732_RS21535 4183321..4183632 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
DS732_RS21540 4183677..4185110 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
DS732_RS21545 4185167..4185910 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T109368 WP_000244777.1 NZ_CP031546:c4180951-4180544 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T109368 NZ_CP031546:c4180951-4180544 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT109368 WP_000354046.1 NZ_CP031546:c4181198-4180932 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT109368 NZ_CP031546:c4181198-4180932 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References