Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3895334..3896131 Replicon chromosome
Accession NZ_CP031546
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name yeeV Uniprot ID A0A346GMF9
Locus tag DS732_RS20035 Protein ID WP_072644276.1
Coordinates 3895754..3896131 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A346GRC6
Locus tag DS732_RS20030 Protein ID WP_072648994.1
Coordinates 3895334..3895708 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS20000 3892756..3892989 + 234 WP_064986988.1 DUF905 family protein -
DS732_RS20010 3893079..3893897 + 819 WP_072644256.1 DUF945 domain-containing protein -
DS732_RS20015 3893988..3894473 + 486 WP_072815258.1 antirestriction protein -
DS732_RS20020 3894489..3894965 + 477 WP_071045793.1 RadC family protein -
DS732_RS20025 3895034..3895255 + 222 WP_000692353.1 DUF987 domain-containing protein -
DS732_RS20030 3895334..3895708 + 375 WP_072648994.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
DS732_RS20035 3895754..3896131 + 378 WP_072644276.1 TA system toxin CbtA family protein Toxin
DS732_RS20040 3896128..3896277 + 150 Protein_3645 hypothetical protein -
DS732_RS20045 3896353..3896550 + 198 WP_094770706.1 DUF957 domain-containing protein -
DS732_RS20050 3896647..3897492 + 846 WP_001280435.1 DUF4942 domain-containing protein -
DS732_RS20060 3898226..3900478 + 2253 Protein_3648 alpha-amylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - stx2B / stx2A 3763545..3917846 154301


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14231.23 Da        Isoelectric Point: 7.7761

>T109365 WP_072644276.1 NZ_CP031546:3895754-3896131 [Escherichia coli]
MKTLPDTYVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNDITQGKHPEVKR

Download         Length: 378 bp

>T109365 NZ_CP031546:3895754-3896131 [Escherichia coli]
ATGAAAACATTACCTGACACATACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGTGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13869.65 Da        Isoelectric Point: 6.2043

>AT109365 WP_072648994.1 NZ_CP031546:3895334-3895708 [Escherichia coli]
VSDKLHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT109365 NZ_CP031546:3895334-3895708 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTATCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTATATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A346GMF9

Antitoxin

Source ID Structure
AlphaFold DB A0A346GRC6

References