Detailed information of TA system TA109364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3604580..3605205
• Replicon: chromosome
• Accession: NZ_CP031546
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DS732_RS18465
• Protein ID: WP_000911330.1
• Coordinates: 3604580..3604978 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: DS732_RS18470
• Protein ID: WP_000450524.1
• Coordinates: 3604978..3605205 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS18445	3600458..3600658	+	201	WP_000383836.1	YpfN family protein	-
DS732_RS18450	3600768..3601466	-	699	WP_000679812.1	esterase	-
DS732_RS18455	3601540..3603555	-	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
DS732_RS18460	3603570..3604433	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
DS732_RS18465	3604580..3604978	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DS732_RS18470	3604978..3605205	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
DS732_RS18475	3605359..3606072	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
DS732_RS18480	3606285..3607319	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
DS732_RS18485	3607336..3608214	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
DS732_RS18490	3608360..3608932	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
DS732_RS18495	3608932..3609402	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T109364 WP_000911330.1 NZ_CP031546:c3604978-3604580 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T109364 NZ_CP031546:c3604978-3604580 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT109364 WP_000450524.1 NZ_CP031546:c3605205-3604978 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT109364 NZ_CP031546:c3605205-3604978 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33