Detailed information of TA system TA109353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2633356..2633882
• Replicon: chromosome
• Accession: NZ_CP031546
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: DS732_RS13425
• Protein ID: WP_000323025.1
• Coordinates: 2633356..2633643 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: DS732_RS13430
• Protein ID: WP_000534858.1
• Coordinates: 2633643..2633882 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS13385	2628380..2628595	-	216	WP_000839590.1	phage lysis protein EssD	-
DS732_RS32100	2628815..2628985	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
DS732_RS13390	2629349..2629564	-	216	WP_000066484.1	cold shock-like protein CspB	-
DS732_RS13395	2629865..2630077	+	213	WP_000087756.1	cold shock-like protein CspF	-
DS732_RS31640	2630132..2630221	+	90	WP_120795389.1	hypothetical protein	-
DS732_RS13400	2630499..2631251	-	753	WP_001047135.1	antitermination protein	-
DS732_RS13405	2631265..2632314	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
DS732_RS13410	2632316..2632594	-	279	WP_012304870.1	hypothetical protein	-
DS732_RS13415	2632661..2632912	-	252	WP_000980994.1	hypothetical protein	-
DS732_RS13420	2633129..2633284	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
DS732_RS13425	2633356..2633643	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
DS732_RS13430	2633643..2633882	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
DS732_RS13435	2633907..2634212	+	306	WP_001326990.1	hypothetical protein	-
DS732_RS13440	2634415..2634747	+	333	WP_001301033.1	protein FlxA	-
DS732_RS13450	2635184..2636497	-	1314	WP_000589005.1	ISNCY family transposase	-
DS732_RS31940	2637266..2637553	-	288	Protein_2450	hypothetical protein	-
DS732_RS13465	2638164..2638520	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2591145..2663883	72738

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T109353 WP_000323025.1 NZ_CP031546:c2633643-2633356 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T109353 NZ_CP031546:c2633643-2633356 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT109353 WP_000534858.1 NZ_CP031546:c2633882-2633643 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT109353 NZ_CP031546:c2633882-2633643 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8