Detailed information of TA system TA109344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 628280..628875
• Replicon: chromosome
• Accession: NZ_CP031546
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: DS732_RS03195
• Protein ID: WP_000239579.1
• Coordinates: 628525..628875 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: DS732_RS03190
• Protein ID: WP_001223208.1
• Coordinates: 628280..628531 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS03140	623657..624193	-	537	WP_077249062.1	DNA-binding protein	-
DS732_RS03145	624223..624523	-	301	Protein_558	hypothetical protein	-
DS732_RS03150	624520..624765	-	246	WP_000153674.1	winged helix-turn-helix domain-containing protein	-
DS732_RS03155	624762..624965	-	204	WP_001560998.1	hypothetical protein	-
DS732_RS31450	625040..625165	-	126	WP_040091107.1	hypothetical protein	-
DS732_RS03160	625162..625404	-	243	WP_000357024.1	DUF4754 family protein	-
DS732_RS03165	625416..625694	-	279	WP_040091109.1	hypothetical protein	-
DS732_RS03170	625705..626055	-	351	WP_064986855.1	DUF4761 family protein	-
DS732_RS03175	626093..626518	-	426	WP_001158283.1	regulatory protein	-
DS732_RS03180	626627..626926	+	300	WP_000108576.1	helix-turn-helix transcriptional regulator	-
DS732_RS03185	626993..627988	+	996	WP_001519231.1	tyrosine-type recombinase/integrase	-
DS732_RS03190	628280..628531	+	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
DS732_RS03195	628525..628875	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
DS732_RS03200	628955..629485	-	531	WP_000055072.1	inorganic diphosphatase	-
DS732_RS03205	629795..630751	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
DS732_RS03215	631061..632563	+	1503	WP_000205793.1	sugar ABC transporter ATP-binding protein	-
DS732_RS03220	632577..633599	+	1023	WP_064986854.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	594932..642683	47751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T109344 WP_000239579.1 NZ_CP031546:628525-628875 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T109344 NZ_CP031546:628525-628875 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT109344 WP_001223208.1 NZ_CP031546:628280-628531 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT109344 NZ_CP031546:628280-628531 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8

Antitoxin

• AlphaFold DB: A0A7U9LQ26