Detailed information of TA system TA109311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1587081..1587831
• Replicon: chromosome
• Accession: NZ_CP031515
• Organism: Dickeya zeae strain EC2

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: DWV07_RS06930
• Protein ID: WP_016943626.1
• Coordinates: 1587081..1587338 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: DWV07_RS06935
• Protein ID: WP_168365339.1
• Coordinates: 1587346..1587831 (+)
• Length: 162 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWV07_RS06920	1583805..1585955	-	2151	WP_038904874.1	formate dehydrogenase subunit alpha	-
DWV07_RS06925	1585990..1586532	-	543	WP_016943624.1	4Fe-4S dicluster domain-containing protein	-
DWV07_RS06930	1587081..1587338	+	258	WP_016943626.1	DUF4160 domain-containing protein	Toxin
DWV07_RS06935	1587346..1587831	+	486	WP_168365339.1	addiction module antitoxin	Antitoxin
DWV07_RS06940	1587920..1588414	-	495	WP_080647337.1	hydrogenase maturation peptidase HycI	-
DWV07_RS06945	1588407..1588805	-	399	WP_168365340.1	formate hydrogenlyase maturation HycH family protein	-
DWV07_RS06950	1588805..1589593	-	789	WP_012885279.1	NADH-quinone oxidoreductase subunit B family protein	-
DWV07_RS06955	1589590..1590159	-	570	WP_038926121.1	hydrogenase 4 subunit H	-
DWV07_RS06960	1590170..1591897	-	1728	WP_168365341.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-73)

Antitoxin

• Uncharacterized protein HI1420 (80-156)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9839.45 Da
• Isoelectric Point: 7.5565

>T109311 WP_016943626.1 NZ_CP031515:1587081-1587338 [Dickeya zeae]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNTKRKLAIAHIMAHREQLLSMWAQAVIGK
NPGKL

Nucleotide

• Length: 258 bp

>T109311 NZ_CP031515:1587081-1587338 [Dickeya zeae]
ATGCCTGAGATTGACAGACTGGCAGGGTTAATCTTCCATCTGTACTTTTACGATGATGAGCGGCACCACTTGCCGCATGT
GCATGTCTGCTACGGCAATCATGAACTTATCATCAGTATTCGTACTGGAACCGAGATAGAGGGATATCTGCCAAATACCA
AACGTAAGCTGGCGATTGCACATATCATGGCGCATCGTGAGCAATTGCTGAGTATGTGGGCACAGGCGGTCATCGGGAAG
AATCCGGGAAAATTGTGA

Antitoxin

Protein

• Length: 162 a.a.
• Molecular weight: 18044.52 Da
• Isoelectric Point: 6.4889

>AT109311 WP_168365339.1 NZ_CP031515:1587346-1587831 [Dickeya zeae]
MYKIVDVDVIGDFSLRLTYDDGLIYDVNLYRYASYPVFLHASQFIRFGLLPDGSLEWPDITISSSELREKGTYVGHTLAV
RERNFADIISRAFYDAVIENRPEILQAALKIAVDKHGNSTVAKEAGAKSRTSLYKSLNKNTNVSLATVVSLAHTVLQLEE
K

Nucleotide

• Length: 486 bp

>AT109311 NZ_CP031515:1587346-1587831 [Dickeya zeae]
ATGTATAAGATTGTGGATGTTGACGTGATTGGCGATTTTTCGTTGCGTCTGACCTATGACGACGGCCTGATATATGACGT
TAACCTGTATCGCTATGCCTCATATCCTGTGTTTTTACATGCCAGCCAATTTATCCGATTTGGGTTATTACCTGATGGTT
CGCTGGAATGGCCGGATATCACGATCTCTTCGTCCGAATTACGGGAAAAGGGGACGTATGTTGGCCACACCCTGGCGGTG
AGGGAGCGGAATTTTGCCGATATCATCTCCCGAGCCTTCTATGATGCCGTGATTGAGAACCGGCCTGAAATTCTGCAGGC
GGCACTGAAAATCGCAGTAGACAAACATGGTAACAGTACGGTGGCCAAAGAGGCGGGAGCCAAAAGCCGTACCAGCTTGT
ATAAATCGCTGAATAAAAATACAAATGTCAGCCTGGCAACGGTGGTTAGCCTGGCCCACACCGTGTTGCAACTAGAAGAG
AAATAG