Detailed information of TA system TA109309

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 720168..720846
• Replicon: chromosome
• Accession: NZ_CP031515
• Organism: Dickeya zeae strain EC2

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: D2BSA8
• Locus tag: DWV07_RS03310
• Protein ID: WP_012883372.1
• Coordinates: 720415..720846 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: DWV07_RS03305
• Protein ID: WP_038906203.1
• Coordinates: 720168..720434 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWV07_RS03290	717319..717927	-	609	WP_168364935.1	HD domain-containing protein	-
DWV07_RS03295	718140..718790	+	651	WP_038913159.1	hemolysin III family protein	-
DWV07_RS03300	718878..719858	-	981	WP_168364936.1	tRNA-modifying protein YgfZ	-
DWV07_RS03305	720168..720434	+	267	WP_038906203.1	FAD assembly factor SdhE	Antitoxin
DWV07_RS03310	720415..720846	+	432	WP_012883372.1	protein YgfX	Toxin
DWV07_RS03315	720935..721453	-	519	WP_016943578.1	flavodoxin FldB	-
DWV07_RS03320	721630..722955	-	1326	WP_038925657.1	MHS family MFS transporter	-
DWV07_RS03325	723219..724118	+	900	WP_016943580.1	site-specific tyrosine recombinase XerD	-
DWV07_RS03330	724205..724921	+	717	WP_019846265.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 16887.54 Da
• Isoelectric Point: 11.5023

>T109309 WP_012883372.1 NZ_CP031515:720415-720846 [Dickeya zeae]
VAQWQCDLRVSWRMQLFSLLTHGFLMLMILLAPWPDGYAPLWLGLVTLVVFDFVRSQRSIKSRQGEISLRGDNQLHWQQR
DWQIVRRPWFMRNGVLLSLRAVDGKGQQRLWLASDSMGSEEWRLLRQQLQQPALHGTESSRHH

Nucleotide

• Length: 432 bp

>T109309 NZ_CP031515:720415-720846 [Dickeya zeae]
GTGGCCCAGTGGCAATGTGATTTACGCGTATCCTGGCGCATGCAGTTGTTTTCGCTGCTAACGCACGGATTTCTGATGCT
GATGATCCTGCTGGCTCCCTGGCCGGACGGCTACGCACCGCTGTGGCTCGGGTTAGTGACGCTGGTCGTATTTGACTTTG
TGCGTAGCCAGCGAAGCATCAAATCGCGCCAGGGGGAGATTTCCCTGCGTGGCGATAACCAACTGCACTGGCAGCAACGT
GACTGGCAGATTGTCCGGCGACCGTGGTTTATGCGCAATGGCGTATTGTTATCGCTGCGTGCGGTGGATGGTAAAGGGCA
ACAGCGTTTGTGGCTGGCGTCAGACAGTATGGGGAGTGAGGAATGGCGGCTGTTACGTCAACAGCTGCAGCAGCCTGCGC
TGCATGGAACGGAATCTTCCCGCCATCATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10446.96 Da
• Isoelectric Point: 5.5981

>AT109309 WP_038906203.1 NZ_CP031515:720168-720434 [Dickeya zeae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDDKQQFIRLLQCDDPDLFNWLMNHGEPVDPGLKRMVSLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT109309 NZ_CP031515:720168-720434 [Dickeya zeae]
ATGGACATCAATAACAAAGCACGTATTCACTGGGCGTGCAGGCGTGGAATGCGCGAACTGGACATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGCTGAGCGACGACGACAAACAGCAGTTTATTCGTCTGCTGCAGTGTGACGATCCTGACT
TGTTTAATTGGTTGATGAACCACGGCGAGCCGGTTGATCCCGGTTTGAAGCGGATGGTTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: A0A2K9QB06