Detailed information of TA system TA109308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4487137..4487830
• Replicon: chromosome
• Accession: NZ_CP031514
• Organism: Chromobacterium sp. Rain0013

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: DXT74_RS20450
• Protein ID: WP_194426894.1
• Coordinates: 4487137..4487514 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: DXT74_RS20455
• Protein ID: WP_194426895.1
• Coordinates: 4487495..4487830 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXT74_RS20405	4482138..4482509	+	372	WP_194426887.1	conjugal transfer transcriptional regulator TraJ	-
DXT74_RS20410	4482546..4483325	+	780	WP_194426888.1	P-type conjugative transfer protein TrbJ	-
DXT74_RS20415	4483339..4483545	+	207	WP_194426889.1	hypothetical protein	-
DXT74_RS20420	4483550..4485106	+	1557	WP_194426890.1	P-type conjugative transfer protein TrbL	-
DXT74_RS20425	4485125..4485379	+	255	WP_194426891.1	hypothetical protein	-
DXT74_RS20430	4485403..4485525	+	123	Protein_3988	conjugal transfer protein TraD	-
DXT74_RS20440	4485729..4485902	+	174	WP_194426892.1	hypothetical protein	-
DXT74_RS20445	4486010..4486972	-	963	WP_194426893.1	hypothetical protein	-
DXT74_RS20450	4487137..4487514	+	378	WP_194426894.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DXT74_RS20455	4487495..4487830	+	336	WP_194426895.1	helix-turn-helix domain-containing protein	Antitoxin
DXT74_RS20460	4487853..4488530	+	678	WP_194426896.1	hypothetical protein	-
DXT74_RS20465	4488527..4489966	+	1440	WP_194426897.1	hypothetical protein	-
DXT74_RS20470	4490302..4491609	+	1308	WP_019103539.1	MFS transporter	-
DXT74_RS20475	4491746..4492063	+	318	WP_019103538.1	DUF883 family protein	-
DXT74_RS20480	4492067..4492456	+	390	WP_081554271.1	phage holin family protein	-
DXT74_RS20485	4492453..4492734	+	282	WP_019103536.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4475143..4498925	23782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13749.89 Da
• Isoelectric Point: 9.5531

>T109308 WP_194426894.1 NZ_CP031514:4487137-4487514 [Chromobacterium sp. Rain0013]
MTEQDRLLIWVGGSKRDLMALPLDVRKFFGHALDFAQRGDQHGAAKPLKGFGGAGVLEIVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKEDMDIIRARLKVAEALAKELRHGKAHD

Nucleotide

• Length: 378 bp

>T109308 NZ_CP031514:4487137-4487514 [Chromobacterium sp. Rain0013]
ATGACAGAACAAGACAGGCTGCTAATCTGGGTCGGAGGAAGTAAACGAGACCTGATGGCGCTTCCTCTCGATGTGCGGAA
GTTCTTTGGTCACGCCCTCGACTTCGCTCAGCGCGGCGACCAGCACGGGGCGGCAAAACCGCTCAAAGGCTTCGGCGGTG
CTGGTGTCCTTGAGATCGTCGAGGACGATGCCGGCGGTACGTACCGGGCCGTCTACACGGTGAAGTTCGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGGATCGCCACGCCGAAAGAGGACATGGACATCATTCGCGCCAG
GCTGAAAGTAGCCGAGGCGCTGGCAAAGGAGCTACGACATGGCAAAGCGCATGATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12195.33 Da
• Isoelectric Point: 9.0957

>AT109308 WP_194426895.1 NZ_CP031514:4487495-4487830 [Chromobacterium sp. Rain0013]
MAKRMIEGIEIETSSGNVFADLGLADAEKLKIKSGLVIEITRAVRRLGLTQEEAGRRMGIPQPKVSGMMRGDFANLSERK
LMECLNRLGYDIEIKVRPAVESIGHLTLAIA

Nucleotide

• Length: 336 bp

>AT109308 NZ_CP031514:4487495-4487830 [Chromobacterium sp. Rain0013]
ATGGCAAAGCGCATGATTGAAGGCATCGAGATCGAAACCAGCTCGGGCAACGTGTTCGCCGACCTCGGCCTGGCCGACGC
CGAAAAGCTCAAGATCAAGTCAGGCCTGGTGATCGAGATCACCCGTGCCGTGCGTCGTCTCGGTTTGACCCAAGAAGAGG
CCGGCCGGCGCATGGGCATCCCACAACCGAAGGTGTCGGGAATGATGCGTGGCGACTTCGCCAACCTCTCCGAACGCAAG
CTCATGGAGTGCCTGAATCGTCTCGGTTACGACATCGAGATCAAGGTTCGGCCGGCCGTCGAGTCGATCGGTCATTTGAC
GCTGGCGATCGCCTGA