Detailed information of TA system TA109304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 5021004..5022592
• Replicon: chromosome
• Accession: NZ_CP031507
• Organism: Dickeya fangzhongdai strain QZH3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: DYD83_RS22210
• Protein ID: WP_100850374.1
• Coordinates: 5021273..5022592 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2K8QTG6
• Locus tag: DYD83_RS22205
• Protein ID: WP_038669098.1
• Coordinates: 5021004..5021273 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYD83_RS22180	5016326..5016937	-	612	WP_100850371.1	NAD(P)-dependent oxidoreductase	-
DYD83_RS22185	5017079..5017459	+	381	WP_100850372.1	helix-turn-helix transcriptional regulator	-
DYD83_RS22190	5017560..5018645	-	1086	WP_038920707.1	4-hydroxyphenylpyruvate dioxygenase	-
DYD83_RS22195	5018961..5019734	+	774	WP_100850373.1	MBL fold metallo-hydrolase	-
DYD83_RS22200	5019784..5020734	+	951	WP_100850517.1	helix-turn-helix domain-containing protein	-
DYD83_RS22205	5021004..5021273	+	270	WP_038669098.1	helix-turn-helix domain-containing protein	Antitoxin
DYD83_RS22210	5021273..5022592	+	1320	WP_100850374.1	type II toxin-antitoxin system HipA family toxin	Toxin
DYD83_RS22215	5022627..5023991	-	1365	WP_100850375.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
DYD83_RS22220	5024107..5025738	-	1632	WP_100850376.1	membrane protein insertase YidC	-
DYD83_RS22225	5025741..5025998	-	258	WP_013320102.1	membrane protein insertion efficiency factor YidD	-
DYD83_RS22230	5025962..5026321	-	360	WP_013320101.1	ribonuclease P protein component	-
DYD83_RS22235	5026340..5026480	-	141	WP_004093983.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49234.37 Da
• Isoelectric Point: 6.5751

>T109304 WP_100850374.1 NZ_CP031507:5021273-5022592 [Dickeya fangzhongdai]
MAALDVYMNGYRVGTLTKTGSGAHHFSYDENWLVLPGSRPISLSMPLRHQPYQGNEVYNFFDNLLPDNPDIRRRIVARHN
ADSTQPFDLLAKVGQDSVGALQLVPQGTPVGDIKQIEYKTLSEQQLENILAGYLADAPLGMIDAEDDFRISIAGAQEKTA
LLYLGDHWCLPFNTTPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCMLVAKAFGLQVPHCFMITAGKIKALAVERFDRK
YASDGRWIMRLPQEDFCQVLNVPSARKYENHGGPGISAIMSYLLGAVDPEKDRYSFMKTQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDRREAKLAMGLTGSRGKKYAIEQIFPRHFFQTAQAVGFARESMESILTEFAQSM
DAVVVNVRNQLPADFPAPIRDAILEGMQARARRLMTGWE

Nucleotide

• Length: 1320 bp

>T109304 NZ_CP031507:5021273-5022592 [Dickeya fangzhongdai]
ATGGCTGCGCTCGATGTTTACATGAACGGCTATCGAGTGGGGACGTTGACCAAAACGGGTAGCGGCGCTCATCATTTTTC
CTATGATGAGAACTGGCTTGTGTTGCCGGGGAGCCGCCCCATCTCGCTCTCGATGCCGCTACGCCATCAGCCCTATCAAG
GTAATGAAGTTTATAATTTCTTCGACAATTTATTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGACATAAC
GCAGATTCTACCCAACCGTTTGACCTGCTGGCTAAAGTGGGGCAGGACAGTGTCGGGGCGTTGCAATTGGTGCCGCAGGG
TACACCTGTTGGCGATATAAAACAGATCGAATACAAAACTTTATCCGAACAACAGCTTGAAAACATACTGGCGGGATACC
TGGCCGATGCGCCACTGGGGATGATTGATGCAGAAGATGATTTTCGTATCTCTATTGCTGGTGCACAGGAAAAGACGGCG
CTGCTCTATCTGGGGGATCACTGGTGTTTGCCCTTCAATACCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCATTCTTACTCAATTGATATGTCTGACAGCGTTGAGAATGAGTATCTGTGCATGCTCGTGGCCAAGGCGT
TTGGGCTGCAGGTACCGCACTGTTTTATGATCACAGCAGGCAAGATAAAAGCGCTGGCGGTAGAACGTTTTGATCGTAAA
TACGCTTCGGATGGTCGTTGGATTATGCGGTTGCCGCAGGAGGATTTTTGCCAGGTATTAAACGTTCCTTCCGCACGGAA
ATACGAAAATCACGGTGGGCCGGGTATTTCCGCCATTATGTCGTATCTATTGGGTGCGGTTGATCCAGAAAAGGATCGTT
ATAGCTTCATGAAGACTCAGGTGCTGTTTTGGCTATTAGCCGCAACAGATGGGCATGCGAAAAATTTTTCCCTGTTTATT
GAATCTGAGGGACGATATCGTCTCACGCCTTTCTATGACATTCTCTCGATGTACCCGGCGATGGGCGGGCGGGGAATTGA
CCGTAGAGAGGCCAAACTGGCGATGGGATTGACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTCCCACGCC
ACTTTTTCCAAACGGCGCAGGCAGTTGGATTTGCCAGAGAATCGATGGAGAGTATTCTGACGGAGTTTGCGCAATCGATG
GATGCTGTTGTGGTGAACGTCAGAAATCAGCTGCCTGCGGATTTTCCTGCACCTATTCGTGATGCGATTCTCGAAGGTAT
GCAGGCCAGGGCAAGGCGATTAATGACCGGGTGGGAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9915.17 Da
• Isoelectric Point: 10.0982

>AT109304 WP_038669098.1 NZ_CP031507:5021004-5021273 [Dickeya fangzhongdai]
MKVTNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTITPRNANAANNEASA
ATSSWKEEW

Nucleotide

• Length: 270 bp

>AT109304 NZ_CP031507:5021004-5021273 [Dickeya fangzhongdai]
ATGAAGGTCACCAACAGCAAACAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACGCAGAAGCTTTCACAAGGAAAAGT
AGCCAGTAAAGTGGGTATTCGCCAGGACACGGTATCCAGCTTCGAACAACACCCTGATTCCACCAAGCTGGAAACATTCT
TTAAAATCCTGTCGGCATTGAATTTAGAACTTACGATTACTCCCAGAAACGCAAATGCCGCCAATAACGAGGCTTCTGCT
GCAACATCATCCTGGAAGGAAGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8QTG6