Detailed information of TA system TA109275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1105266..1105792
• Replicon: chromosome
• Accession: NZ_CP031505
• Organism: Dickeya fangzhongdai strain LN1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: C6CCH1
• Locus tag: DYD82_RS05085
• Protein ID: WP_015854715.1
• Coordinates: 1105266..1105571 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: DYD82_RS05090
• Protein ID: WP_100849039.1
• Coordinates: 1105574..1105792 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYD82_RS05075	1101869..1103878	+	2010	WP_188726081.1	ATP-dependent helicase	-
DYD82_RS05080	1103880..1105139	+	1260	WP_100849038.1	GIY-YIG nuclease family protein	-
DYD82_RS05085	1105266..1105571	-	306	WP_015854715.1	type II toxin-antitoxin system toxin CcdB	Toxin
DYD82_RS05090	1105574..1105792	-	219	WP_100849039.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
DYD82_RS05095	1105851..1106594	-	744	WP_100849040.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
DYD82_RS05100	1107496..1107846	-	351	WP_100849042.1	hypothetical protein	-
DYD82_RS05105	1107969..1108157	+	189	WP_033111580.1	hypothetical protein	-
DYD82_RS05110	1108310..1109572	-	1263	WP_100849043.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1091377..1109749	18372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11676.54 Da
• Isoelectric Point: 4.8616

>T109275 WP_015854715.1 NZ_CP031505:c1105571-1105266 [Dickeya fangzhongdai]
MQFIVYEYKRASHYKMFVDVQSDIVETPKRRMAIPLIESHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVI
ADLGGYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T109275 NZ_CP031505:c1105571-1105266 [Dickeya fangzhongdai]
ATGCAATTCATTGTTTATGAATACAAACGCGCCAGTCATTACAAAATGTTTGTCGATGTGCAAAGTGATATTGTCGAGAC
TCCCAAGCGGCGTATGGCTATCCCGCTTATCGAATCGCACCACCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCGCTGA
TTCGCATCGATGGGGAAGATTATCGGCTGATGACCACTGAGCTATCGAGCGTTCCTGTTGAGGTCATTGGTGAAGTTATC
GCGGATCTTGGCGGCTACGCAGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8137.12 Da
• Isoelectric Point: 8.6801

>AT109275 WP_100849039.1 NZ_CP031505:c1105792-1105574 [Dickeya fangzhongdai]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEAWRKENREGMEEVARLIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT109275 NZ_CP031505:c1105792-1105574 [Dickeya fangzhongdai]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGCATCAAAGCTGAAGCGTGGAGAAAGGAAAACCGCGAAGGGATGGAGG
AAGTCGCCCGACTCATCGCACAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2K8QIT0