Detailed information of TA system TA109271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 422414..423249
• Replicon: chromosome
• Accession: NZ_CP031505
• Organism: Dickeya fangzhongdai strain LN1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: DYD82_RS01970
• Protein ID: WP_100848750.1
• Coordinates: 422875..423249 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: DYD82_RS01965
• Protein ID: WP_100848749.1
• Coordinates: 422414..422875 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYD82_RS01955	418260..418553	+	294	WP_012883104.1	type I-E CRISPR-associated endoribonuclease Cas2	-
DYD82_RS01960	420190..421866	-	1677	WP_100848748.1	MCP four helix bundle domain-containing protein	-
DYD82_RS01965	422414..422875	+	462	WP_100848749.1	helix-turn-helix domain-containing protein	Antitoxin
DYD82_RS01970	422875..423249	+	375	WP_100848750.1	toxin PIN	Toxin
DYD82_RS01975	423473..423925	+	453	WP_038917619.1	YhbP family protein	-
DYD82_RS01980	424037..424501	-	465	WP_100848751.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
DYD82_RS01985	424600..426738	-	2139	WP_100848752.1	anaerobic ribonucleoside-triphosphate reductase	-
DYD82_RS01990	427118..428026	+	909	WP_100848753.1	homocysteine S-methyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SinI-like, DNA-binding domain (84-131)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13806.82 Da
• Isoelectric Point: 4.6730

>T109271 WP_100848750.1 NZ_CP031505:422875-423249 [Dickeya fangzhongdai]
MNQAIPDAMITGFEALIPGITLPDPDDQHVVAAAIRSNAEIIVTFNLKDFPHPEIDNFGIEALHPDDFITDLLDLNQALV
LSAVSRQRHSMRKPPKSVDEYLDALLRQSLPQTVKELSKFHALI

Nucleotide

• Length: 375 bp

>T109271 NZ_CP031505:422875-423249 [Dickeya fangzhongdai]
ATGAATCAGGCTATTCCTGATGCGATGATTACTGGGTTTGAAGCGCTCATTCCAGGCATCACGCTCCCTGATCCTGATGA
TCAGCATGTGGTTGCAGCCGCCATTCGCAGTAACGCTGAAATCATCGTTACGTTTAACCTGAAAGATTTTCCCCATCCCG
AGATCGATAACTTTGGCATTGAAGCGCTACACCCGGATGATTTCATCACAGACCTGCTCGATTTGAATCAGGCGCTTGTG
TTATCCGCAGTAAGCAGGCAGCGTCACAGCATGAGAAAACCGCCTAAATCGGTTGATGAGTATCTTGATGCGTTACTCCG
GCAGAGCTTACCGCAAACAGTAAAAGAATTGAGTAAATTCCACGCCCTGATTTAG

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16883.25 Da
• Isoelectric Point: 6.2221

>AT109271 WP_100848749.1 NZ_CP031505:422414-422875 [Dickeya fangzhongdai]
MTNSILDSLSLPAPGEIEAAVRGQRELAAYLSTKMETQKISIQDADNNTRQVELPTSALTLLMSILGELAVGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGKLPFHKTGRHRRVLFADLMDYKHQRDNESSRAMQALADHGQELGLY

Nucleotide

• Length: 462 bp

>AT109271 NZ_CP031505:422414-422875 [Dickeya fangzhongdai]
ATGACAAACTCAATTCTTGATAGCCTGAGCCTTCCGGCACCGGGGGAGATTGAGGCGGCTGTTCGTGGACAAAGGGAGCT
TGCTGCCTATCTCTCCACAAAGATGGAAACACAAAAAATCTCGATTCAGGATGCAGATAACAACACTCGTCAGGTCGAAT
TACCGACGTCGGCACTGACATTATTGATGTCGATTTTAGGCGAGCTGGCTGTGGGTAACGCGGTTCAGGTTGTTCCTGTT
CATGCTGAATTAACGACACAGGAGGCAGCCAACATTCTGAATGTTTCACGCCCTCACATGGTGAAGCTTCTGGAGGATGG
GAAACTACCTTTTCATAAAACCGGTCGCCACCGCCGTGTGCTTTTCGCTGACCTGATGGACTATAAACACCAGAGGGATA
ATGAAAGCAGTAGAGCAATGCAGGCGTTGGCAGATCATGGCCAAGAGCTTGGATTGTACTGA