Detailed information of TA system TA109270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/VapC(toxin)
• Location: 2263010..2263644
• Replicon: chromosome
• Accession: NZ_CP031500
• Organism: Deinococcus radiodurans strain R1 dM1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9RX96
• Locus tag: DXG80_RS11730
• Protein ID: WP_010887064.1
• Coordinates: 2263231..2263644 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DXG80_RS11725
• Protein ID: WP_162177573.1
• Coordinates: 2263010..2263234 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXG80_RS11690	2258160..2258843	+	684	WP_010887056.1	SDR family oxidoreductase	-
DXG80_RS11695	2258864..2259826	+	963	WP_034349507.1	NAD(P)/FAD-dependent oxidoreductase	-
DXG80_RS11700	2259849..2260028	+	180	WP_034349506.1	hypothetical protein	-
DXG80_RS11705	2260030..2260659	+	630	WP_027479568.1	HAD family hydrolase	-
DXG80_RS11710	2260707..2262188	-	1482	Protein_2215	carboxylesterase/lipase family protein	-
DXG80_RS11715	2262368..2262610	+	243	WP_010887061.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DXG80_RS11720	2262604..2262957	+	354	WP_010887062.1	endoribonuclease MazF	-
DXG80_RS11725	2263010..2263234	+	225	WP_162177573.1	hypothetical protein	Antitoxin
DXG80_RS11730	2263231..2263644	+	414	WP_010887064.1	type II toxin-antitoxin system VapC family toxin	Toxin
DXG80_RS11735	2263641..2266109	+	2469	WP_010887065.1	ATP-dependent helicase HrpB	-
DXG80_RS11740	2266290..2266514	-	225	WP_162177572.1	hypothetical protein	-
DXG80_RS11745	2266670..2267446	-	777	WP_010887067.1	trans-aconitate 2-methyltransferase	-
DXG80_RS11750	2267567..2267746	-	180	WP_028328008.1	hypothetical protein	-
DXG80_RS11755	2267743..2268369	-	627	WP_010887068.1	single-stranded DNA-binding protein DdrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14634.68 Da
• Isoelectric Point: 5.5820

>T109270 WP_010887064.1 NZ_CP031500:2263231-2263644 [Deinococcus radiodurans]
MTESTVLDANILSALLRSEANPLSIARQLSALAREGSLVVYAELLAGPEVTPDFLKGFLQEVDVQILPPSGLAVWESAGL
AYGSYAQRRQRSGGGSPRQILSDFVIGAHALYHGAALFTADPQHYRLSFPALTVLTP

Nucleotide

• Length: 414 bp

>T109270 NZ_CP031500:2263231-2263644 [Deinococcus radiodurans]
ATGACAGAGAGCACCGTGCTGGACGCCAATATTCTCAGTGCCCTGCTCCGTTCTGAGGCGAACCCTCTGTCCATTGCGCG
TCAGCTTTCGGCGCTGGCCCGTGAGGGTTCACTAGTGGTTTATGCCGAGTTGCTCGCGGGCCCTGAGGTTACGCCCGATT
TCCTCAAGGGATTTCTGCAAGAGGTGGACGTGCAGATTCTTCCGCCGTCTGGCCTTGCGGTCTGGGAAAGCGCGGGCCTC
GCTTATGGGAGCTACGCTCAGCGGCGGCAACGCAGCGGTGGTGGGTCGCCTCGCCAGATTCTGAGTGACTTTGTGATTGG
TGCCCATGCCCTGTATCACGGCGCCGCTCTCTTCACCGCTGATCCTCAGCATTACCGCCTCAGCTTCCCAGCCCTCACAG
TCCTGACCCCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8229.23 Da
• Isoelectric Point: 4.3481

>AT109270 WP_162177573.1 NZ_CP031500:2263010-2263234 [Deinococcus radiodurans]
MTTLEARIENGKVVLTPEQAAELGLREGEVLPVTIAPHEAETEGNPFTRWIGTLPPLPDGEESARFYRRLRDGE

Nucleotide

• Length: 225 bp

>AT109270 NZ_CP031500:2263010-2263234 [Deinococcus radiodurans]
ATGACGACGTTGGAGGCCCGGATTGAGAACGGCAAGGTGGTGCTCACCCCTGAGCAGGCCGCCGAACTGGGCCTGCGCGA
AGGCGAAGTGCTGCCGGTGACGATTGCGCCGCACGAAGCGGAAACGGAAGGCAACCCCTTCACCCGCTGGATTGGGACGC
TCCCCCCACTGCCTGACGGTGAAGAGTCTGCGCGGTTCTACCGCCGCCTGCGAGACGGCGAATGA

Structures

Toxin

• AlphaFold DB: Q9RX96